RT Journal Article SR Electronic(1) A1 Wang, Xiang A1 Jiang, Wan-Kui A1 Cui, Meng-Di A1 Yang, Zhan-Gong A1 Yu, Xing A1 Hu, Gang A1 Zhang, Hao A1 Hong, QingYR 2017 T1 Nocardioides agrisoli sp. nov., isolated from farmland soil JF International Journal of Systematic and Evolutionary Microbiology, VO 67 IS 10 SP 3722 OP 3727 DO https://doi.org/10.1099/ijsem.0.002158 PB Microbiology Society, SN 1466-5034, AB A novel Gram-stain-positive bacterium, designated djl-8T, was isolated from farmland soil in Nanjing, Jiangsu province, PR China. Cells of strain djl-8T were aerobic, non-motile, non-spore-forming and rod-shaped. The organism grew at 25–37 °C, pH 5.5–8.0 and 0.5–4.0 % NaCl (w/v). The DNA G+C content was 69.3 mol%. The diagnostic diamino acid in the cell-wall peptidoglycan was LL-2, 6-diaminopimelic acid. The major fatty acids (>5 %) were iso-C16 : 0, anteiso-C17 : 0, iso-C15 : 0, 10-Me C17 : 0 and C17 : 1ω8c. The respiratory quinone was MK-8 (H4) and the major polar lipids were phosphatidylglycerol, phosphatidylinositol, diphosphatidylglycerol and unknown phospholipids. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain djl-8T is a member of the genus Nocardioides and shared the highest similarity with Nocardioides ginkgobilobae SYP-A7303T (97.1 %), followed by Nocardioides soli mbc-2T (96.9 %), Nocardioide spyridinolyticus OS4T (96.6 %) and Nocardioides maradonensis RP-B30T (96.6 %). Strain djl-8T exhibited low DNA–DNA relatedness with Nocardioides ginkgobilobae SYP-A7303T (26.9±2.1 %). On the basis of the morphological, physiological, biochemical and chemotaxonomic characteristics presented in this study, strain djl-8T represents a novel species of the genus Nocardioides , for which the name Nocardioides agrisoli sp. nov. is proposed. The type strain is djl-8T (=KCTC 39844T=CCTCC AB 2017058T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002158