1887

Abstract

A novel Gram-stain-negative bacterial strain, designated T16R-256, was isolated from the rhizosphere soil of tomato plants grown in a greenhouse in Yecheon-gun, Gyeongsangbuk-do, Republic of Korea and characterized using polyphasic taxonomy. Cells were aerobic, non-flagellated and rod-shaped. Colonies were light yellow, convex and round. The strain grew in the temperature range of 15–37 °C (optimally at 28–30 °C) and pH range of 7.0–9.0 (optimally at 7.0–8.0) and in 4 % NaCl (w/v). A comparison of 16S rRNA gene sequences showed that strain T16R-256 is a member of the genus , exhibiting high sequence similarity with P-4 (94.2 %), RK1 (93.7 %), Jip14 (93.7 %), 4M29 (93.6 %) and DCY14 (93.4 %). The major polar lipids were phosphatidylethanolamine, sphingolipid, one aminophospholipid, two aminolipids and three unknown lipids. The major fatty acids (>10 % of the total fatty acids) were iso-C, iso-C 3-OH and iso-C 2-OH/Cω7. Strain T16R-256 contained MK-7 as the predominant respiratory quinone and homospermidine as the major polyamine. The genomic DNA G+C content of the type strain was 55.5 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain T16R-256 should be designated as representative of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is T16R-256 (=KACC 18788=JCM 31602).

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2017-10-01
2024-03-29
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