RT Journal Article SR Electronic(1) A1 Wang, Hai-liang A1 Zhang, Jian A1 Sun, LiYR 2017 T1 Bacillus iocasae sp. nov., isolated from Pacmanus hydrothermal field, Manus Basin JF International Journal of Systematic and Evolutionary Microbiology, VO 67 IS 9 SP 3547 OP 3552 DO https://doi.org/10.1099/ijsem.0.002164 PB Microbiology Society, SN 1466-5034, AB A novel bacterial strain S36T was isolated from the deep-sea sediment collected from Pacmanus hydrothermal field, Manus Basin. The strain was Gram-stain-positive, aerobic, rod-shaped, endospore-forming, and motile. It was able to grow at 16–50 °C, pH 6.0–10.0, and in the presence of 0–11 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain S36T was a member of genus Bacillus and shares the highest sequence identity with Bacillus herbersteinensis D-1,5aT (97.0 %). The value of DNA–DNA hybridization between strain S36T and B. herbersteinensis D-1,5aT was 22.8 %. The cell wall diagnostic diamino acid of strain S36T was meso-diaminopimelic acid and the polar lipid profile of strain S36T contained diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. The predominant respiratory quinine was MK-7. The major cellular fatty acids were iso-C15 : 0 and anteiso-C15 : 0. The genomic DNA G+C content of strain S36T was 43.0 mol%. On the basis of phylogenetic analysis, DNA–DNA hybridization, and phenotypic characteristics, it was concluded that strain S36T represents a novel species of the genus Bacillus , for which the name Bacillus iocasae sp. nov. was proposed. The type strain is S36T (=KCTC 33864T=DSM 104297T=CGMCC 1.16030T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002164