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Volume 67, Issue 10

gen. nov., sp. nov., isolated from a tidal flat Free

Abstract

A Gram-stain-negative, strictly aerobic, moderately halophilic bacterium, designated A-1, was isolated from a tidal flat of the Taean coast in South Korea. Cells were motile rods with a single flagellum showing oxidase-negative and catalase-positive activities and contained poly--hydroxyalkanoic acid granules. Growth of strain A-1 was observed at 20–40 °C (optimum, 30 °C), pH 6.0–10.5 (optimum, pH 7.0) and in the presence of 1.0–6.0 % (w/v) NaCl (optimum, 2.0 %). Strain A-1 contained C, summed feature 3 (comprising Cω7 and/or Cω6) and summed feature 8 (comprising C 7 and/or C 6) as the major fatty acids. The major polar lipids of strain A-1 were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The isoprenoid quinones detected were ubiquinone-7 and ubiquinone-8. The G+C content of the genomic DNA was 51.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain A-1 formed a distinct phylogenetic lineage from other genera within the family . Strain A-1 shared low 16S rRNA gene sequence similarities with other taxa (≤94.9 %). On the basis of phenotypic, chemotaxonomic and molecular properties, it is clear that strain A-1 represents a novel genus and species of the family , for which the name gen. nov., sp. nov. is proposed. The type strain is A-1 (=KACC 18119=JCM 30136).

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Keyword(s): new taxa , Oceanospirillaceae , PHA , Pontibacterium granulatum and tidal fla
© 2017 IUMS
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2017-10-01
2024-04-19
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Pontibacterium granulatum gen. nov., sp. nov., isolated from a tidal flat
Int J Syst Evol Microbiol 67, 3784 (2017); https://doi.org/10.1099/ijsem.0.002190
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