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Volume 67, Issue 10

sp. nov., isolated from bark of Free

Abstract

A Gram-stain negative, aerobic, non-motile bacterial strain, 23D10-4-9, was isolated from symptomatic canker bark tissue of . The isolate grew between 4 and 35 °C, with optimal growth occurring at 25 °C. The species was positive for catalase and negative for oxidase activity. Nitrate was not reduced to nitrite. It showed activities toward -galactosidase and -glucosidase. Citrate was not utilized. Acid was produced from -glucose. The major fatty acids were iso-C, Cω and iso-C 3-OH. The main polar lipid profiles of the novel isolate included phosphatidylethanolamine, phospholipids and seven unknown lipids. The predominant menaquinone of the novel isolate was MK-7. The DNA G+C content was 40.6 mol%. 16S rRNA gene data revealed that the novel isolate shares the greatest sequence similarity with 7Y-4 (96.1 %). Based on phenotypic and genotypic characteristics, the isolate represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is 23D10-4-9 (=CFCC 12640=KCTC 42248).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): bark , Populus × euramericana and Sphingobacterium corticis sp. nov.
© 2017 IUMS
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2017-10-01
2024-04-20
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References

  1. Yabuuchi E, Kaneko T, Yano I, Moss CW, Miyoshi N. Sphingobacterium gen. nov., Sphingobacterium spiritivorum comb. nov., Sphingobacterium multivorum comb. nov., Sphingobacterium mizutae sp. nov., and Flavobacterium indologenes sp. nov.: glucose-nonfermenting Gram-negative rods in CDC groups IIK-2 and IIb. Int J Syst Bacteriol 1983; 33:580–598 [View Article]
     [Google Scholar]
  2. Li Y, He W, Ren F, Guo L, Chang J et al. A canker disease of Populus × euramericana in China caused by Lonsdalea quercina subsp. populi . Plant Disease 2014; 98:368–378 [View Article]
     [Google Scholar]
  3. Baker GC, Smith JJ, Cowan DA. Review and re-analysis of domain-specific 16S primers. J Microbiol Methods 2003; 55:541–555 [View Article][PubMed]
     [Google Scholar]
  4. Lane DJ. 16S/23S rRNA sequencing. In Stackebrandt E, Goodfellow M. (editors) Nucleic Acid Techniques in Bacterial Systematics Chichester: Wiley; 1991 pp. 115–175
     [Google Scholar]
  5. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article][PubMed]
     [Google Scholar]
  6. Li Y, Song LM, Guo MW, Wang LF, Liang WX. Description of Sphingobacterium populi sp. nov., isolated from bark of Populus × euramericana . Int J Syst Evol Microbiol 2016; 66:3456–3462 [View Article][PubMed]
     [Google Scholar]
  7. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article][PubMed]
     [Google Scholar]
  8. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011; 28:2731–2739 [View Article][PubMed]
     [Google Scholar]
  9. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997; 25:4876–4882 [View Article][PubMed]
     [Google Scholar]
  10. Gomori G. Preparation of buffers for use in enzyme studies. In Colowick SP, Kaplan NO. (editors) Methods in Enzymology vol. 274 NewYork: Academic Press; 1955 pp. 138–146
     [Google Scholar]
  11. Smibert RM, Krieg NR. Phenotypic characterization. In Gerhardt P, Murray RGE, Wood WA, Krieg NR. (editors) Manual of Methods for General and Microbiology Washington, DC: American Society for Microbiology; 1994 pp. 607–654
     [Google Scholar]
  12. Jenkins D, Richard MG, Daigger GT. Manual on the Causes and Control of Activated Sludge Bulking and Foaming Water Research Commission; 1986
     [Google Scholar]
  13. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Notes 101. Newark, DE: MIDI Inc; 1990
     [Google Scholar]
  14. Nichols PD, Guckert JB, White DC. Determination of monosaturated fatty acid double-bond position and geometry for microbial monocultures and complex consortia by capillary GC-MS of their dimethyl disulphide adducts. J Microbiol Methods 1986; 5:49–55 [View Article]
     [Google Scholar]
  15. Collins MD, Pirouz T, Goodfellow M, Minnikin DE. Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 1977; 100:221–230 [View Article][PubMed]
     [Google Scholar]
  16. Du HJ, Zhang YQ, Liu HY, Su J, Wei YZ et al. Allonocardiopsis opalescens gen. nov., sp. nov., a new member of the suborder Streptosporangineae, from the surface-sterilized fruit of a medicinal plant. Int J Syst Evol Microbiol 2013; 63:900–904 [View Article][PubMed]
     [Google Scholar]
  17. Groth I, Schumann P, Rainey FA, Martin K, Schuetze B et al. Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. Int J Syst Bacteriol 1997; 47:1129–1133 [View Article][PubMed]
     [Google Scholar]
  18. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
     [Google Scholar]
  19. Komagata K, Suzuki K. Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 1987; 19:161–207 [Crossref]
     [Google Scholar]
  20. Marmur J. A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 1961; 3:208–IN1 [View Article]
     [Google Scholar]
  21. Mesbah M, Premachandran U, Whitman WB. Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 1989; 39:159–167 [View Article]
     [Google Scholar]
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Sphingobacterium corticis sp. nov., isolated from bark of Populus × euramericana
Int J Syst Evol Microbiol 67, 3860 (2017); https://doi.org/10.1099/ijsem.0.002210
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