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Volume 67, Issue 10

Classification of sp. ATCC 33076, an actinomycete that produces the glycolipodepsipeptide antibiotic ramoplanin, as sp. nov. Free

Abstract

Strain ATCC 33076, which produces the antibiotic ramoplanin, was isolated from a soil sample collected in India, and it was classified as a member of the genus on the basis of morphology and cell-wall composition. A phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain forms a distinct clade within the genus , and it is most closely related to IFO 13994 (98.71 % similarity) and Y16 (98.33 %). The strain forms an extensively branched substrate mycelium; the sporangia are formed very scantily and are globose with irregular surface. Spores are oval and motile. The cell wall contains -diaminopimelic acid and the diagnostic sugars are xylose and arabinose. The predominant menaquinone is MK-9(H), with minor amounts of MK-9(H) and MK-9(H). Mycolic acids are absent. The diagnostic phospholipids are phosphatidylethanolamine, hydroxyphosphatidylethanolamine and phosphatidylglycerol. The major cellular fatty acids are anteiso-C and iso-C, followed by iso-C and moderate amounts of anteiso-C, iso-C and C 9. The genomic DNA G+C content is 71.4 mol%. Significant differences in the morphological, chemotaxonomic and biochemical data, together with DNA–DNA relatedness between strain ATCC 33076 and closely related type strains, clearly demonstrated that strain ATCC 33076 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is ATCC 33076 (=DSM 105064=NRRL B-65484).

  • Received:
  • Accepted:
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Keyword(s): actinomycete , Actinoplanes ramoplaninifer sp. nov. , Actinoplanes sp. ATCC 33076 , antibiotics and ramoplanin
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2017-10-01
2024-04-26
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Classification of Actinoplanes sp. ATCC 33076, an actinomycete that produces the glycolipodepsipeptide antibiotic ramoplanin, as Actinoplanes ramoplaninifer sp. nov.
Int J Syst Evol Microbiol 67, 4181 (2017); https://doi.org/10.1099/ijsem.0.002281
/content/journal/ijsem/10.1099/ijsem.0.002281
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