1887

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Volume 67, Issue 10

sp. nov., isolated from rhizosphere soil of L., and emended descriptions of the species and Free

Abstract

Two Gram-stain-positive, aerobic actinomycete strains, designated NEAU-PCY-3 and NEAU-PCY-4, were isolated from rhizosphere soil of L. collected from Anshan, Liaoning Province, northeast PR China. The 16S rRNA gene sequence analysis showed that the two strains exhibited 99.9 % 16S rRNA gene sequence similarity with each other and that they were most closely to DSM 45356 (98.7, 98.9 %) and JCM 11711 (97.1, 97.2 %). Phylogenetic analysis based on the 16S rRNA gene sequences indicated that the two strains were located in the same lineage and formed a cluster with the genus Both strains were observed to contain MK-10(H) and MK-10(H) as the predominant menaquinones. The cell wall peptidoglycan was found to contain -diaminopimelic acid, -glutamic acid, glycine and -alanine. Whole-cell hydrolysates mainly contained galactose, ribose and xylose. The phospholipid profile contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, several glycolipids and several unknown lipids. The major cellular fatty acids for strain NEAU-PCY-3 were iso-C, iso-C, anteiso-C and C 5. The DNA–DNA hybridization value between strains NEAU-PCY-3 and NEAU-PCY-4 was 83.6±0.4 %, and the values between the two strains and their closest phylogenetic relatives, belonging to the genus were well below 70 %, supporting that they represented a distinct genomic species. An array of phenotypic characteristics also differentiated the strains from their closely related species, the only two validly published species. On the basis of the genetic, chemotaxonomic and phenotypic properties, strains NEAU-PCY-3 and NEAU-PCY-4 were classified as representatives of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NEAU-PCY-3 (=DSM 105119=CCTCC AA 2017017).

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Keyword(s): 16S rRNA gene , Longispora urticae sp. nov. and polyphasic taxonomy
© 2017 IUMS
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2017-10-01
2024-04-26
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Longispora urticae sp. nov., isolated from rhizosphere soil of Urtica urens L., and emended descriptions of the species Longisporaalbida and Longisporafulva
Int J Syst Evol Microbiol 67, 4228 (2017); https://doi.org/10.1099/ijsem.0.002288
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