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Volume 67, Issue 11

sp. nov., a bacterium isolated from soil Free

Abstract

A Gram-reaction-negative, aerobic, non-motile, white (translucent) and rod-shaped bacterium (designated HKS-06) isolated from soil was characterized by a polyphasic approach to clarify its taxonomic position. Strain HKS-06 was observed to grow optimally at 30 °C and at pH 6.5–7.0 on R2A agar medium. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain HKS-06 belongs to the genus and is most closely related to JS5 (97.4 % similarity). The G+C content of the genomic DNA was 64.1 mol%. Chemotaxonomic data [major quinone (Q-10), major polar lipids (phosphatidylethanolamine, phosphatidylglycerol, sphingoglycolipid, phosphatidylcholine, unknown polar lipid) and major fatty acids (summed feature 8, comprising Cω7cω6 and/or Cω6, C 3-OH and C)] supported the affiliation of strain HKS-06 to the genus . Moreover, the physiological and biochemical results and low level of DNA–DNA relatedness [between strain HKS06 and . JS5 (20.24±1.2 %)] allowed the phenotypic and genotypic differentiation of strain HKS-06 from recognized species of the genus . The new isolate therefore represents a novel species, for which the name sp. nov. is proposed. The type strain is HKS-06 (=KACC 18880=LMG 29563).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA gene sequence , polyphasic taxonomy , soil and Sphingomonas agri
© 2017 IUMS
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2017-11-01
2024-04-19
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Sphingomonas agri sp. nov., a bacterium isolated from soil
Int J Syst Evol Microbiol 67, 4429 (2017); https://doi.org/10.1099/ijsem.0.002306
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