1887

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Volume 68, Issue 1

sp. nov., isolated from a dairy cow with mastitis Free

Abstract

Here we describe a new species of the genus that was isolated from a dairy cow with mastitis in New Zealand. Strain NZ1587 was Gram-positive, coccus-shaped and arranged as chains, catalase and coagulase negative, γ-haemolytic and negative for Lancefield carbohydrates (A-D, F and G). The 16S rRNA sequence did not match sequences in the NCBI 16S rRNA or GreenGenes databases. Taxonomic classification of strain NZ1587 was investigated using 16S rRNA and core genome phylogeny, genome-wide average nucleotide identity (ANI) and predicted DNA–DNA hybridisation (DDH) analyses. Phylogeny based on 16S rRNA was unable to resolve the taxonomic position of strain NZ1587, however NZ1587 shared 99.4 % identity at the 16S rRNA level with a distinct branch of Importantly, core genome phylogeny demonstrated that NZ1587 grouped amongst the ‘pyogenic’ streptococcal species and formed a distinct branch supported by a 100 % bootstrap value. In addition, average nucleotide identity and inferred DNA–DNA hybridisation analyses showed that NZ1587 represents a novel species. Biochemical profiling using the rapid ID 32 strep identification test enabled differentiation of strain NZ1587 from closely related streptococcal species. In conclusion, strain NZ1587 can be classified as a novel species, and we propose a novel taxon named sp. nov.; the type strain is NZ1587. NZ1587 has been deposited in the Culture Collection University of Gothenburg (CCUG 69277) and the Belgian Co-ordinated Collections of Micro-organisms/LMG (LMG 29747).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): bovine , mastitis , pathogen and Streptococcus
© 2018 IUMS
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2018-01-01
2024-04-18
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References

  1. Gao XY, Zhi XY, Li HW, Klenk HP, Li WJ. Comparative genomics of the bacterial genus Streptococcus illuminates evolutionary implications of species groups. PLoS One 2014; 9:e101229 [View Article][PubMed]
     [Google Scholar]
  2. Bradley A. Bovine mastitis: an evolving disease. Vet J 2002; 164:116–128 [View Article][PubMed]
     [Google Scholar]
  3. Denis M, Wedlock DN, Lacy-Hulbert SJ, Hillerton JE, Buddle BM. Vaccines against bovine mastitis in the New Zealand context: what is the best way forward?. N Z Vet J 2009; 57:132–140 [View Article][PubMed]
     [Google Scholar]
  4. Phillips EA, Tapsall JW, Smith DD. Rapid tube CAMP test for identification of Streptococcus agalactiae (Lancefield group B). J Clin Microbiol 1980; 12:135–137[PubMed]
     [Google Scholar]
  5. Khot PD, Couturier MR, Wilson A, Croft A, Fisher MA. Optimization of matrix-assisted laser desorption ionization-time of flight mass spectrometry analysis for bacterial identification. J Clin Microbiol 2012; 50:3845–3852 [View Article][PubMed]
     [Google Scholar]
  6. Quail MA, Kozarewa I, Smith F, Scally A, Stephens PJ et al. A large genome center's improvements to the Illumina sequencing system. Nat Methods 2008; 5:1005–1010 [View Article][PubMed]
     [Google Scholar]
  7. Page AJ, de Silva N, Hunt M, Quail MA, Parkhill J et al. Robust high-throughput prokaryote de novo assembly and improvement pipeline for Illumina data. Microb Genom 2016; 2:e000083 [View Article][PubMed]
     [Google Scholar]
  8. Seemann T. Prokka: rapid prokaryotic genome annotation. Bioinformatics 2014; 30:2068–2069 [View Article][PubMed]
     [Google Scholar]
  9. Schalm OW, Noorlander DO. Experiments and observations leading to development of the california mastitis test. J Am Vet Med Assoc 1957; 130:199–204[PubMed]
     [Google Scholar]
  10. Katoh K, Standley DM. MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Mol Biol Evol 2013; 30:772–780 [View Article][PubMed]
     [Google Scholar]
  11. Stamatakis A. RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 2006; 22:2688–2690 [View Article][PubMed]
     [Google Scholar]
  12. Shewmaker PL, Steigerwalt AG, Whitney AM, Morey RE, Graziano JC et al. Evaluation of methods for identification and determination of the taxonomic status of strains belonging to the Streptococcus porcinus-Streptococcus pseudoporcinus complex isolated from animal, human, and dairy sources. J Clin Microbiol 2012; 50:3591–3597 [View Article][PubMed]
     [Google Scholar]
  13. Stackebrant E, Elbers J. Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 2006; 33:152–155
     [Google Scholar]
  14. Bekal S, Gaudreau C, Laurence RA, Simoneau E, Raynal L et al. Streptococcus pseudoporcinus sp. nov., a novel species isolated from the genitourinary tract of women. J Clin Microbiol 2006; 44:2584–2586 [View Article][PubMed]
     [Google Scholar]
  15. Duarte RS, Barros RR, Facklam RR, Teixeira LM. Phenotypic and genotypic characteristics of Streptococcus porcinus isolated from human sources. J Clin Microbiol 2005; 43:4592–4601 [View Article][PubMed]
     [Google Scholar]
  16. Page AJ, Cummins CA, Hunt M, Wong VK, Reuter S et al. Roary: rapid large-scale prokaryote pan genome analysis. Bioinformatics 2015; 31:3691–3693 [View Article][PubMed]
     [Google Scholar]
  17. Richter M, Rosselló-Móra R, Oliver Glöckner F, Peplies J. JSpeciesWS: a web server for prokaryotic species circumscription based on pairwise genome comparison. Bioinformatics 2016; 32:929–931 [View Article]
     [Google Scholar]
  18. Meier-Kolthoff JP, Auch AF, Klenk HP, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013; 14:60 [View Article][PubMed]
     [Google Scholar]
  19. Konstantinidis KT, Tiedje JM. Genomic insights that advance the species definition for prokaryotes. Proc Natl Acad Sci USA 2005; 102:2567–2572 [View Article][PubMed]
     [Google Scholar]
  20. Wayne LG, Moore WEC, Stackebrandt E, Kandler O, Colwell RR et al. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Evol Microbiol 1987; 37:463–464 [View Article]
     [Google Scholar]
  21. Mende DR, Sunagawa S, Zeller G, Bork P. Accurate and universal delineation of prokaryotic species. Nat Methods 2013; 10:881–884 [View Article][PubMed]
     [Google Scholar]
  22. Fortin M, Messier S, Paré J, Higgins R. Identification of catalase-negative, non-beta-hemolytic, gram-positive cocci isolated from milk samples. J Clin Microbiol 2003; 41:106–109 [View Article][PubMed]
     [Google Scholar]
  23. Freney J, Bland S, Etienne J, Desmonceaux M, Boeufgras JM et al. Description and evaluation of the semiautomated 4-hour rapid ID 32 Strep method for identification of streptococci and members of related genera. J Clin Microbiol 1992; 30:2657–2661[PubMed]
     [Google Scholar]
  24. Lau SK, Curreem SO, Lin CC, Fung AM, Yuen KY et al. Streptococcus hongkongensis sp. nov., isolated from a patient with an infected puncture wound and from a marine flatfish. Int J Syst Evol Microbiol 2013; 63:2570–2576 [View Article][PubMed]
     [Google Scholar]
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Streptococcus bovimastitidis sp. nov., isolated from a dairy cow with mastitis
Int J Syst Evol Microbiol 68, 21 (2018); https://doi.org/10.1099/ijsem.0.002321
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