@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.002328, author = "Fang, Xiao-Mei and Yan, Dong and Bai, Jing-Lin and Su, Jing and Liu, Hong-Yu and Ma, Bai-Ping and Zhang, Yu-Qin and Yu, Li-Yan", title = "Ornithinimicrobium flavum sp. nov., isolated from the leaf of Paris polyphylla", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2017", volume = "67", number = "11", pages = "4541-4545", doi = "https://doi.org/10.1099/ijsem.0.002328", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002328", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "CPCC 203535", keywords = "Ornithinimicrobium flavum", keywords = "Paris polyphylla", keywords = "new taxa-actinobacteria", abstract = "A Gram-positive bacterium originating from the surface-sterilized leaf of Paris polyphylla var. yunnanensis (Franch.) was characterized by using a polyphasic approach. The isolate formed yellow, smooth, circular colonies on nutrient agar with 0.2 % starch (NSA). Cells were non-motile, non-sporulating, irregular rods or cocci. Strain CPCC 203535T had the highest 16S rRNA gene sequence similarity to the type strain of Ornithinimicrobium kibberense (96.9 %) and formed the deepest branch in the genus Ornithinimicrobium in the neighbour-joining (NJ) phylogenetic tree based on 16S rRNA gene sequences. The major menaquinones of strain CPCC 203535T were MK-8(H4), MK-8(H2) and MK-8. The peptidoglycan contained ornithine as the diagnostic diamino acid. The polar lipid profile consisted of diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylinositol (PI) and unknown lipid (UL). The major fatty acids iso-C14 : 0, iso-C15 : 0, iso-C16 : 0 and anteiso-C15 : 0 were consistent with the fatty acid patterns reported for members of the genus Ornithinimicrobium . The DNA G+C composition is 71.4 mol%. The results of physiological and biochemical tests allowed phenotypic differentiation of strain CPCC 203535T from its closest phylogenetic species in the genus Ornithinimicrobium . Strain CPCC 203535T represents a novel species of the genus Ornithinimicrobium , for which the name Ornithinimicrobium flavum sp. nov. is proposed, with CPCC 203535T (=NBRC 109452 T=KCTC 29164T) as the type strain.", }