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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 67, Issue 11

gen. nov., sp. nov., a moderately thermophilic bacterium isolated from a hot spring Free

Abstract

A Gram-stain-negative, ovoid-shaped, aerobic, non-motile, catalase- and oxidase-positive, and moderately thermophilic bacterial strain, designated strain YIM 72346, was isolated from a sediment sample collected from a hot spring in Tengchong county, Yunnan province, south-west China. Growth occurred at 37–50 °C (optimum, 45 °C), at pH 6.0–9.0 (optimum, pH 6.5–7.0) and in the presence of 0.5–1.0 % (w/v) NaCl (optimum, 0.5 %). The major cellular fatty acids were Cω7, C, Ccyclo 8,and C 2-OH. The genomic DNA G+C content was determined to be 69.8 mol%. The predominant ubiquinone was Q-10. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, one unidentified aminolipid and two unidentified phospholipids. Bacteriochlorophyll and carotenoic acids were not detected. Strain YIM 72346 was not observed for the accumulation of poly--hydroxybutyrate. The strain shared highest 16S rRNA gene sequence identities with YIM 78023 (93.3 %) and NS130 (92.7 %), but formed a distinct lineage within the family in the phylogenetic trees. On the basis of genotypic, phenotypic, chemotaxonomic and phylogenetic analyses, strain YIM 72346 is considered to represent a novel genus and species of the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is YIM 72346 (=KCTC 52714=CGMCC 1.16330).

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Keyword(s): Caldovatus sediminis gen. nov., sp. nov. , hot spring , slightly thermophilic and Tengchong
© 2017 IUMS
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2017-11-01
2024-04-25
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Caldovatus sediminis gen. nov., sp. nov., a moderately thermophilic bacterium isolated from a hot spring
Int J Syst Evol Microbiol 67, 4716 (2017); https://doi.org/10.1099/ijsem.0.002363
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