%0 Journal Article %A Fang, Bao-Zhu %A Hua, Zheng-Shuang %A Han, Ming-Xian %A Zhang, Zi-Tong %A Wang, Yi-Huan %A Yang, Zi-Wen %A Zhang, Wan-Qin %A Xiao, Min %A Li, Wen-Jun %T Nonomuraea cavernae sp. nov., a novel actinobacterium isolated from a karst cave sample %D 2017 %J International Journal of Systematic and Evolutionary Microbiology, %V 67 %N 11 %P 4692-4697 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.002364 %K Nonomuraea cavernae sp. nov. %K karst cave %K polyphasic taxonomy %I Microbiology Society, %X A novel actinobacterial strain, designated SYSU K10005T, was isolated from a soil sample collected from a karst cave in Xingyi county, Guizhou province, south-west China. The taxonomic position of the strain was investigated using a polyphasic approach. Cells of the strain were aerobic and Gram-stain-positive. On the basis of 16S rRNA gene sequence analysis, strain SYSU K10005T was most closely related to the type strains of the genus Nonomuraea , and shared highest sequence similarity of 98.4 % with Nonomuraea candida HMC10T. DNA–DNA hybridization values between the two strains were less than 70 %. The whole-cell hydrolysates of strain SYSU K10005T contained meso-diaminopimelic acid (diagnostic diamino acid), and arabinose, madurose and rhamnose (whole-cell sugars). The major isoprenoid quinone was MK-9(H4), while the major fatty acids were iso-C16 : 0, 10-methyl C17 : 0, C17  : 1ω8c and C17 : 0. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, hydroxyl-phosphatidylethanolamine, lyso-phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides, an unidentified lipid, two unidentified ninhydrin-positive phosphoglycolipids and two unidentified phospholipids. The genomic DNA G+C content of strain SYSU K10005T was 64.2 mol%. On the basis of phenotypic, genotypic and phylogenetic data, strain SYSU K10005T can be characterized to represent a novel species of the genus Nonomuraea , for which the name Nonomuraea cavernae sp. nov. is proposed. The type strain is SYSU K10005T (=KCTC 39805T=CGMCC 4.7368T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002364