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Volume 67, Issue 11

gen. nov., sp. nov., a novel betaproteobacterium isolated from a waste-treating bioreactor Free

Abstract

A new betaproteobacterium, CGI-09, was isolated from an activated sludge bioreactor which treated landfill leachate. Based on 16S rRNA gene sequence analysis, the new strain shared the highest pairwise similarity values with members of the order : IAM 13946 (family ), 93.7 % and DSM 11362 (family ), 93.6 %. Cells of strain CGI-09 were rod-shaped and non-motile. The new strain was oxidase and catalase positive and capable of reducing nitrate to nitrite. The predominant fatty acids were C ω7, C, cycloC and C ω7, the major respiratory quinone was Q-8, and the detected polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and an unknown phospholipid. The G+C content of the genomic DNA of strain CGI-09 was 70.2 mol%. The new bacterium can be distinguished from the members of genera and based on its non-motile cells, arginine dihydrolase activity, its high cyclo C fatty acid content and the lack of hydroxy fatty acids. On the basis of the phenotypic, chemotaxonomic and molecular data, strain CGI-09 is considered to represent a new genus and species within the family , for which the name gen. nov., sp. nov. is proposed. The type strain is CGI-09 (=DSM 29781=JCM 31785).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): activated sludge , class Betaproteobacteria , new genus and Quisquiliibacterium
© 2017 IUMS
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2017-11-01
2024-04-20
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Quisquiliibacterium transsilvanicum gen. nov., sp. nov., a novel betaproteobacterium isolated from a waste-treating bioreactor
Int J Syst Evol Microbiol 67, 4742 (2017); https://doi.org/10.1099/ijsem.0.002368
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