1887

Abstract

A novel actinomycete, designated strain 1H-SSA8, was isolated from the head of an ant ( Mayr) and was found to produce amphotericin. A polyphasic approach was employed to determine the status of strain 1H-SSA8. Morphological and chemotaxonomic characteristics were consistent with those of members of the genus . The menaquinones detected were MK-9(H), MK-9(H) and MK-9(H). The phospholipid profile consisted of diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine and phosphatidylinositol mannoside. The major fatty acids were identified as iso-C, C, C and anteiso-C. Analysis of the 16S rRNA gene sequence showed that strain 1H-SSA8 belongs to the genus with high sequence similarity to NRRL B-2714 (99.2 %). Two tree-making algorithms based on 16S rRNA gene sequences showed that the isolate formed a phyletic line with ATCC 53653 (98.7 %). The MLSA utilizing partial sequences of the housekeeping genes (D, B, A, B and B) also supported the position. However, evolutionary distances were higher than the 0.007 MLSA evolutionary distance threshold proposed for species-level relatedness. Moreover, the low level of DNA–DNA relatedness and phenotypic differences allowed the novel isolate to be differentiated from its most closely related strain NRRL B-2714 and strain ATCC 53653. It is concluded that the organism can be classified as representing a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 1H-SSA8 (=CGMCC 4.7350=DSM 103128).

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2017-12-01
2024-03-28
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