1887

Abstract

Two novel strains, J116-2 and J116-1, were isolated from forest soil and were taxonomically characterized by a polyphasic approach. Both strains were yellow-coloured, Gram-stain-negative, strictly aerobic, non-motile and rod-shaped bacteria. The strains were non-sporulating, catalase-positive and oxidase-negative. Strains J116-2 and J116-1 were able to grow at 20–32 °C, pH 6.0–8.5, and 0–0.5 % (w/v) NaCl concentration. Based on 16S rRNA gene sequence analyses, strains J116-2 and J116-1 formed a distinct lineage within the family of the phylum and were closely related to genera (89.86–89.30 % sequence similarity), (89.20–89.06 %), (89.06 %) and (89.01–88.77 %). The pairwise sequence similarity between strains J116-2 and J116-1 was found to be 99.86 %. Flexirubin-type pigments were absent in both strains. The only respiratory quinone was menaquinone-7 (MK-7); the major polar lipid was phosphatidylethanolamine; the predominant polyamine was homospermidine; and the major fatty acids were Ciso, Ciso G and Ciso 3-OH. The genomic DNA G+C content values of strains J116-2 and J116-1 were 51.1 and 50.9 mol%, respectively. On the basis of phenotypic, genotypic and phylogenetic analysis, strain J116-2 represents a novel species of a new genus in the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is J116-2 (=KEMB 9005–550=KACC 19168=NBRC 112827).

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2018-02-01
2024-03-28
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