1887

Abstract

A pale-brown-coloured, rod-shaped, non-motile, and Gram-reaction-negative bacterium, strain I54, was isolated from a water sample of a freshwater river in Iksan, Republic of Korea. The phylogenetic affiliation based on 16S rRNA gene sequence analysis showed that strain I54 belonged to the genus of the family with a sequence similarity of 97.5 % to HM0024. The major fatty acids (>5 %) of strain I54 were iso-C, anteiso-C, summed feature 3 (C 7/Cω6) and iso-C 3-OH. Phosphatidylethanolamine, one aminolipid and two unknown lipids were the major polar lipids. The G+C content of the genomic DNA was 34.2 (±0.3)mol% and MK6 was the sole respiratory quinone. On the basis of its molecular and phenotypic characteristics, strain I54 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is I54 (=KCTC 52722=JCM 31880).

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2017-12-01
2024-04-26
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References

  1. Joung Y, Song J, Lee K, Oh HM, Joh K et al. Soonwooa buanensis gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from seawater. Int J Syst Evol Microbiol 2010; 60:2061–2065 [View Article][PubMed]
    [Google Scholar]
  2. Soto-Rodriguez SA, Cabanillas-Ramos J, Alcaraz U, Gomez-Gil B, Romalde JL. Identification and virulence of Aeromonas dhakensis, Pseudomonas mosselii and Microbacterium paraoxydans isolated from Nile tilapia, Oreochromis niloticus, cultivated in Mexico. J Appl Microbiol 2013; 115:654–662 [View Article][PubMed]
    [Google Scholar]
  3. Kim HJ, Yong HI, Lee HJ, Jung S, Kwon JH et al. Identification of microorganisms in duck meat products available in korea and the effect of high hydrostatic pressure. Korean J Food Sci Anim Resour 2016; 36:283–288 [View Article][PubMed]
    [Google Scholar]
  4. Padakandla SR, Lee GW, Chae JC. Paenibacillus gelatinilyticus sp. nov. a psychrotolerant bacterium isolated from a reclaimed soil and amended description of Paenibacillus shenyangensis . Antonie van Leeuwenhoek 2015; 108:1197–1203 [View Article][PubMed]
    [Google Scholar]
  5. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article][PubMed]
    [Google Scholar]
  6. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425[PubMed]
    [Google Scholar]
  7. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971; 20:406–416 [View Article]
    [Google Scholar]
  8. Edwards AWF, Cavalli-Sforza LL. The reconstruction of evolution. Heredity 18; 553 (Abstract) Ann Hum Genet 1963; 27:105
    [Google Scholar]
  9. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; 30:2725–2729 [View Article][PubMed]
    [Google Scholar]
  10. Kimura M. The Neutral Theory of Molecular Evolution Cambridge: Cambridge University Press; 1983 [Crossref]
    [Google Scholar]
  11. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article][PubMed]
    [Google Scholar]
  12. Tindall BJ, Rosselló-Móra R, Busse HJ, Ludwig W, Kämpfer P. Notes on the characterization of prokaryote strains for taxonomic purposes. Int J Syst Evol Microbiol 2010; 60:249–266 [View Article][PubMed]
    [Google Scholar]
  13. Bernardet JF, Nakagawa Y, Holmes B. Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 2002; 52:1049–1070 [View Article][PubMed]
    [Google Scholar]
  14. Buck JD. Nonstaining (KOH) method for determination of gram reactions of marine bacteria. Appl Environ Microbiol 1982; 44:992–993[PubMed]
    [Google Scholar]
  15. Yang SJ, Cho JC. Gaetbulibacter marinus sp. nov., isolated from coastal seawater, and emended description of the genus Gaetbulibacter . Int J Syst Evol Microbiol 2008; 58:315–318 [View Article][PubMed]
    [Google Scholar]
  16. Hameed A, Shahina M, Lin SY, Sridhar KR, Young LS et al. Siansivirga zeaxanthinifaciens gen. nov., sp. nov., a novel zeaxanthin-producing member of the family Flavobacteriaceae isolated from coastal seawater of Taiwan. FEMS Microbiol Lett 2012; 333:37–45 [View Article][PubMed]
    [Google Scholar]
  17. Bauer AW, Kirby WM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 1966; 45:493–496[PubMed]
    [Google Scholar]
  18. Cappuccino JG, Sherman N. Microbiology – A Laboratory Manual, 4th ed. San Francisco, CA: Benjamin/Cummings Science Publishing; 1998
    [Google Scholar]
  19. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
    [Google Scholar]
  20. Tindall BJ, Sikorski J, Smibert RM, Kreig NR. Phenotypic characterization and the principles of comparative systematics. In Reddy CA, Beveridge TJ, Breznak JA, Marzluf G, Schmidt TM et al. (editors) Methods for General and Molecular Microbiology, 3rd ed. Washington, DC: ASM Press; 2007 pp. 330–393
    [Google Scholar]
  21. Kates M. Techniques of Lipidology New York: Elsevier; 1972 [Crossref]
    [Google Scholar]
  22. Oren A, Duker S, Ritter S. The polar lipid composition of Walsby's square bacterium. FEMS Microbiol Lett 1996; 138:135–140 [View Article]
    [Google Scholar]
  23. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc; 2001
    [Google Scholar]
  24. Tamaoka J. Analysis of bacterial menaquinone mixtures by reverse-phase high-performance liquid chromatography. Methods Enzymol 1986; 123:31–36[PubMed]
    [Google Scholar]
  25. Marmur J. A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 1961; 3:208–218 [View Article]
    [Google Scholar]
  26. Mesbah M, Premachandran U, Whitman WB. Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 1989; 39:159–167 [View Article]
    [Google Scholar]
  27. Kim M, Oh HS, Park SC, Chun J. Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. Int J Syst Evol Microbiol 2014; 64:346–351 [View Article][PubMed]
    [Google Scholar]
  28. Meier-Kolthoff JP, Göker M, Spröer C, Klenk HP. When should a DDH experiment be mandatory in microbial taxonomy?. Arch Microbiol 2013; 195:413–418 [View Article][PubMed]
    [Google Scholar]
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