@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.002443, author = "Sheu, Shih-Yi and Su, Ching-Ling and Kwon, Soon-Wo and Chen, Wen-Ming", title = "Flavobacterium amniphilum sp. nov., isolated from a stream", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2017", volume = "67", number = "12", pages = "5179-5186", doi = "https://doi.org/10.1099/ijsem.0.002443", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002443", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Bacteroidetes", keywords = "new taxa", keywords = "polyphasic taxonomy", keywords = "stream", keywords = "Flavobacterium amniphilum", keywords = "Flavobacteriaceae", abstract = "Strain KYPY10T, isolated from a water sample taken from the Funglin Stream in Taiwan, was characterized using a polyphasic taxonomic approach. Cells of strain KYPY10T were Gram-stain-negative, strictly aerobic, motile by gliding, rod-shaped surrounded by a thick capsule, and formed light-yellow colonies. Growth occurred at 20–30 °C (optimum, 25 °C), at pH 6–7 (optimum, pH 6) and with 0–0.2 % NaCl (optimum, 0 % w/v). Phylogenetic analyses, based on 16S rRNA gene sequences, showed that strain KYPY10T belonged to the genus Flavobacterium and was most closely related to Flavobacterium brevivitae TTM-43T (98.4 % sequence similarity) and Flavobacterium vireti THG-SM1T (98.0 %). Strain KYPY10T contained iso-C15 : 0, iso-C15 : 1 G, summed feature 9 (iso-C17 : 1ω9c and/or 10-methyl C16 : 0), iso-C17 : 0 3-OH, iso-C15 : 0 3-OH and iso-C16 : 0 as the predominant fatty acids. The major isoprenoid quinone was MK-6. The polar lipid profile consisted of phosphatidylethanolamine, four uncharacterized aminophospholipids, five uncharacterized phospholipids and one uncharacterized lipid. The major polyamines were homospermidine and putrescine. The genomic DNA G+C content of strain KYPY10T was 41.0 mol%. The DNA–DNA relatedness of strain KYPY10T with respect to recognized species of the genus Flavobacterium was less than 70 %. On the basis of the phylogenetic inference and phenotypic data, strain KYPY10T was recognized as a representative of a novel species within the genus Flavobacterium . The name Flavobacterium amniphilum sp. nov. is proposed, with strain KYPY10T (=BCRC 81006T=LMG 29727T=KCTC 52443T) as the type strain.", }