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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 67, Issue 12

gen. nov., sp. nov., isolated from farmland soil during development of new culture techniques Free

Abstract

Two novel strains, J77-1 and J76-1, were isolated from farmland soil and were taxonomically characterized by a polyphasic approach. Both strains were yellow, Gram-stain-negative, strictly aerobic, non-motile and rod-shaped bacteria. These strains were non-sporulating, catalase-positive and oxidase-negative. J77-1 and J76-1 were able to grow at 15–40 °C, pH 5.0–10.0, and 0–1.0 % (w/v) NaCl concentration. Phylogenetic analyses revealed that both strains formed a distinct separate lineage within the family of the phylum . J77-1 and J76-1 showed low 16S rRNA gene sequence similarity to the most closely related type strain WPCB159 (85.09 %) and exhibited less than 85.0 % sequence similarity with other members of the family . The pairwise sequence similarity between strains J77-1 and J76-1 was observed to be 99.86 %. In both strains, the only respiratory quinone was menaquinone-7 (MK-7); the major polar lipid was phosphatidylethanolamine; and the major fatty acids were summed feature 3 (Cω7 and/or Cω6), C, iso-C, iso-C 3-OH Cω5, and C 3-OH. The genomic DNA G+C content values of J77-1 and J76-1 were 50.1 and 50.9 mol%, respectively. On the basis of the results of phenotypic, genotypic and phylogenetic analysis, J77-1 represents a novel species of a novel genus, for which the name gen. nov., sp. nov. is proposed, within the family . The type strain of is J77-1 (=KEMB 9005-548=KACC 19172=JCM 31920), and J76-1 is an additional strain.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Bacteroidetes , Cytophagaceae , Cytophagales , farmland soil , new culture techniques and Ravibacter arvi
© 2017 IUMS
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2017-12-01
2024-04-16
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Ravibacter arvi gen. nov., sp. nov., isolated from farmland soil during development of new culture techniques
Int J Syst Evol Microbiol 67, 5252 (2017); https://doi.org/10.1099/ijsem.0.002456
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