1887

Abstract

A Gram-stain-positive, facultatively anaerobic bacterial strain, GDSW-R2A3, was isolated from a downstream water sample collected from the river Ganges, India. Analysis of the 16S rRNA gene sequence of strain GDSW-R2A3 revealed its affiliation to the family . Further analysis using a polyphasic approach revealed that strain GDSW-R2A3 was most closely related to the genus . Analysis of the almost-complete (1488 bp) 16S rRNA gene sequence of strain GDSW-R2A3 revealed the highest level of sequence similarity with CCM 8426 (98.3 %) and KCTC 13712 (98.3 %) followed by DSM 14730 (98.0 %). The digital DNA–DNA hybridization and average nucleotide identity (ANI) values between strain GDSW-R2A3 and the most closely related taxon, CCM 8426, were 20.3 and 78.2 %, respectively. The DNA G+C content of the strain was 44.2 mol%. The cell-wall amino acid was -diaminopimelic acid. Polar lipids present were phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, three aminophospholipids, two phospholipids and one unidentified lipid; the major menaquinone was MK-7; iso-C, iso-C and anteiso-C were the major fatty acids. On the basis of the phenotypic, chemotaxonomic and phylogenetic data, it can be concluded that strain GDSW-R2A3 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is GDSW-R2A3 (=VTCC-B-910015=CCM 8782).

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2018-01-01
2024-03-28
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