@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.002474, author = "Pal, Deepika and Bhardwaj, Ayanka and Kaur, Navjot and Sudan, Sarabjeet Kour and Bisht, Bhawana and Kumari, Munesh and Vyas, Bhawna and Krishnamurthi, Srinivasan and Mayilraj, Shanmugam", title = "Fictibacillus aquaticus sp. nov., isolated from downstream river water", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2018", volume = "68", number = "1", pages = "160-164", doi = "https://doi.org/10.1099/ijsem.0.002474", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002474", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "16S rRNA gene", keywords = "Fictibacillus", keywords = "fatty acid methyl esters", keywords = "dDDH", abstract = "A Gram-stain-positive, facultatively anaerobic bacterial strain, GDSW-R2A3T, was isolated from a downstream water sample collected from the river Ganges, India. Analysis of the 16S rRNA gene sequence of strain GDSW-R2A3T revealed its affiliation to the family Bacillaceae . Further analysis using a polyphasic approach revealed that strain GDSW-R2A3T was most closely related to the genus Fictibacillus . Analysis of the almost-complete (1488 bp) 16S rRNA gene sequence of strain GDSW-R2A3T revealed the highest level of sequence similarity with Fictibacillus phosphorivorans CCM 8426T (98.3 %) and Fictibacillus nanhaiensis KCTC 13712T (98.3 %) followed by Fictibacillus barbaricus DSM 14730T (98.0 %). The digital DNA–DNA hybridization and average nucleotide identity (ANI) values between strain GDSW-R2A3T and the most closely related taxon, F. phosphorivorans CCM 8426T, were 20.3 and 78.2 %, respectively. The DNA G+C content of the strain was 44.2 mol%. The cell-wall amino acid was meso-diaminopimelic acid. Polar lipids present were phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, three aminophospholipids, two phospholipids and one unidentified lipid; the major menaquinone was MK-7; iso-C14 : 0, iso-C15 : 0 and anteiso-C15 : 0 were the major fatty acids. On the basis of the phenotypic, chemotaxonomic and phylogenetic data, it can be concluded that strain GDSW-R2A3T represents a novel species of the genus Fictibacillus , for which the name Fictibacillus aquaticus sp. nov. is proposed. The type strain is GDSW-R2A3T (=VTCC-B-910015T=CCM 8782T).", }