RT Journal Article SR Electronic(1) A1 Park, Jisun A1 Kim, Min-Kyeong A1 Yun, Bo-Ram A1 Han, Ji-Hye A1 Kim, Seung BumYR 2018 T1 Pseudogracilibacillus endophyticus sp. nov., a moderately thermophilic and halophilic species isolated from plant root JF International Journal of Systematic and Evolutionary Microbiology, VO 68 IS 1 SP 165 OP 169 DO https://doi.org/10.1099/ijsem.0.002475 PB Microbiology Society, SN 1466-5034, AB A Gram-stain-positive strain, designated DT7-02T, was isolated from the surface-sterilized root of Oenotherabiennis (evening primrose) and subjected to taxonomic characterization. Cells of DT7-02T were slender rod-shaped, motile by means of flagella, and oxidase- and catalase-positive. The colonies were circular, pinkish-yellow, opaque, glistering and 1–2 mm in diameter. The strain was moderately thermophilic and halophilic, as growth occurred at 20–44 °C (optimum 40 °C), pH 7–10 (optimum pH 8–9) and in the presence of 0–8 % of NaCl (optimum 4 %) in tryptic soy broth. The analysis of 16S rRNA gene sequences indicated that the strain represented a member of the genus Pseudogracilibacillus of the family Bacillaceae , and the sequence similarity was 96.5 % with Pseudogracilibacillus auburnensis P-207T and 95.9 % with Pseudogracilibacillus marinus NIOT-bflm-S4T. Other related taxa were Ornithinibacillus contaminans DSM 22953T and Sinibacillus soli KCTC 33117T, with 16S rRNA gene sequence similarities of 95.4 and 94.3 %, respectively. The major cellular fatty acids of DT7-02T were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0. The DNA G+C content was 35.1 mol%, and the respiratory quinone was MK-7. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol and phosphatidylethanolamine. The combination of chemotaxonomic properties enabled differentiation of DT7-02T from the other two species of the genus Pseudogracilibacillus . The results of phylogenetic, phenotypic and chemotaxonomic analyses demonstrate that strain DT7-02T (=KCTC 33854T=JCM 31192T) merits recognition as representing a novel species of the genus Pseudogracilibacillus , for which the name Pseudogracilibacillus endophyticus sp. nov. is proposed., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002475