1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 68, Issue 1

Genetic diversity and symbiotic effectiveness of strains nodulating selected annual grain legumes growing in Ethiopia Free

Abstract

, and growing in Ethiopia are nodulated by a genetically diverse group of strains. To determine the genetic identity and symbiotic effectiveness of these bacteria, a collection of 36 test strains originating from the root nodules of the three hosts was investigated using multilocus sequence analyses (MLSA) of core genes including 16S rRNA, , , , and . Sequence analysis of and genes along with tests for symbiotic effectiveness using δN analysis were also carried out. The phylogenetic trees derived from the MLSA grouped most test strains into four well-supported distinct positions designated as genospecies I–IV. The maximum likelihood (ML) tree that was constructed based on the gene sequences separated the entire test strains into two lineages, where the majority of the test strains were clustered on one of a well-supported large branch that comprise species from the tropics. This clearly suggested the monophyletic origin of the genes within the bradyrhizobia of tropical origin. The δN-based symbiotic effectiveness test of seven selected strains revealed that strains GN100 (δ =0.73) and GN102 (δ =0.79) were highly effective nitrogen fixers when inoculated to cowpea, thus can be considered as inoculants in cowpea production. It was concluded that Ethiopian soils are a hotspot for rhizobial diversity. This calls for further research to unravel as yet unknown bradyrhizobia nodulating legume host species growing in the country. In this respect, prospective research should also address the mechanisms of symbiotic specificity that could lead to high nitrogen fixation in target legumes.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Bradyrhizobium , Ethiopia , Genospecies , MLSA and symbiotic effectiveness
© 2018 IUMS
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2018-01-01
2024-04-27
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Genetic diversity and symbiotic effectiveness of Bradyrhizobium strains nodulating selected annual grain legumes growing in Ethiopia
Int J Syst Evol Microbiol 68, 449 (2018); https://doi.org/10.1099/ijsem.0.002486
/content/journal/ijsem/10.1099/ijsem.0.002486
/content/journal/ijsem/10.1099/ijsem.0.002486
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