1887

Abstract

During a study of petroleum hydrocarbon-degrading bacteria, a yellow-coloured, Gram-stain-negative, non-motile and rod-shaped bacterium, designated strain Brt-M, was isolated from oil-contaminated soil of Biratnagar, Morang, Nepal. Strain Brt-M was able to grow at 15–45 °C, pH 5.0–9.0 and 0–1 % (w/v) NaCl concentration. The strain was characterized by multiple taxonomic approaches. Based on 16S rRNA gene sequence analysis, strain Brt-M belonged to the genus and shared highest sequence similarity with wh15 (95.69 %) and MK3 (94.91 %). The only respiratory quinone was MK-6; the major polar lipid was phosphatidylethanolamine; and the predominant fatty acids were iso-C, summed feature 9 (iso-Cω9 and/or C10-methyl), iso-C 3-OH and summed feature 3 (Cω7 and/or Cω6). The DNA G+C content was 37.2 mol%. The morphological, physiological, chemotaxonomic and phylogenetic analyses clearly distinguished this strain from its closest phylogenetic neighbours. Thus, strain Brt-M represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Brt-M (=KEMB 9005-692=KACC 19393=JCM 32171).

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2018-01-01
2024-03-28
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