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Volume 68, Issue 1

gen. nov., sp. nov., isolated from a lake water sample, and proposal of fam. nov. within the order Free

Abstract

A -like bacterium, designated strain SYSU HZH-2, was isolated from a water sample collected from Haizhu Lake, Guangzhou, China. The bacterium was fastidious, and required an exogenous source of -cysteine for its growth on artificial media. Cells were Gram-stain-negative, coccobacilli, non-motile and non-spore-forming. The strain shared highest 16S rRNA gene sequence similarities with SYSU D3-2 (94.6 % identity), UST040201-002 (93.2 %) and 51 (91.6 %). This strain possessed ubiquinone-8 as the respiratory quinone; diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine and phosphatidylglycerol as the known polar lipids, and anteiso-C and C as the major fatty acids (>10 % of total fatty acids). The dendrograms based on 16S rRNA gene sequence analysis showed that it formed a separate cluster along with SYSU D3-2, 51 and UST040201-002. Based on the 16S rRNA gene sequence identity and differences in other phenotypic characteristics, the strain is considered to represent a novel species of a novel genus, for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is SYSU HZH-2 (=NBRC 112274 = CGMCC 1.15950). Additionally, the new taxon along with the genera , and (family unassigned) were distinctly separated from the related families , and in the phylogenetic trees. Therefore, we proposed a new family fam. nov. within the order to accommodate these four genera.

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  • Accepted:
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Keyword(s): Fastidiosibacter lacustris gen. nov., sp. nov. , Fastidiosibacteraceae fam. nov. and Haizhu Lake
© 2018 IUMS
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2018-01-01
2024-04-20
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References

  1. Qu P, Deng X, Zhang J, Chen J, Zhang J et al. Identification and characterization of the Francisella sp. strain 08HL01032 isolated in air condition systems. Wei Sheng Wu Xue Bao 2009; 49:1003–1010[PubMed]
     [Google Scholar]
  2. Qu PH, Chen SY, Scholz HC, Busse HJ, Gu Q et al. Francisella guangzhouensis sp. nov., isolated from air-conditioning systems. Int J Syst Evol Microbiol 2013; 63:3628–3635 [View Article][PubMed]
     [Google Scholar]
  3. Qu PH, Li Y, Salam N, Chen SY, Liu L et al. Allofrancisella inopinata gen. nov., sp. nov. and Allofrancisella frigidaquae sp. nov., isolated from water-cooling systems, and transfer of Francisella guangzhouensis Qu et al. 2013 to the new genus as Allofrancisella guangzhouensis comb. nov. Int J Syst Evol Microbiol 2016; 66:4832–4838 [View Article][PubMed]
     [Google Scholar]
  4. Cui XL, Mao PH, Zeng M, Li WJ, Zhang LP et al. Streptimonospora salina gen. nov., sp. nov., a new member of the family Nocardiopsaceae . Int J Syst Evol Microbiol 2001; 51:357–363 [View Article][PubMed]
     [Google Scholar]
  5. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1618 [View Article][PubMed]
     [Google Scholar]
  6. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol 1990; 215:403–410 [View Article][PubMed]
     [Google Scholar]
  7. Liu L, Salam N, Jiao JY, E SM, Chen C et al. Cysteiniphilum litorale gen. nov., sp. nov., isolated from coastal seawater. Int J Syst Evol Microbiol 2017; 67:2178–2183 [View Article][PubMed]
     [Google Scholar]
  8. Liu L, Zhou EM, Jiao JY, Manikprabhu D, Ming H et al. Hymenobacter mucosus sp. nov., isolated from a karst cave soil sample. Int J Syst Evol Microbiol 2015; 65:4121–4127 [View Article][PubMed]
     [Google Scholar]
  9. Bonetta S, Bonetta S, Ferretti E, Balocco F, Carraro E. Evaluation of Legionella pneumophila contamination in Italian hotel water systems by quantitative real-time PCR and culture methods. J Appl Microbiol 2010; 108:1576–1583 [View Article][PubMed]
     [Google Scholar]
  10. Xu P, Li WJ, Tang SK, Zhang YQ, Chen GZ et al. Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family 'Oxalobacteraceae' isolated from China. Int J Syst Evol Microbiol 2005; 55:1149–1153 [View Article][PubMed]
     [Google Scholar]
  11. Aslanzadeh J. Biochemical profile-based microbial identification systems [M]. In Tang YW, Stratton CW. (editors) Advanced Techniques in Diagnostic Microbiology New York: Springer; 2013 pp. 87–121 [Crossref]
     [Google Scholar]
  12. Minnikin DE, Collins MD, Goodfellow M. Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol 1979; 47:87–95 [Crossref]
     [Google Scholar]
  13. Collins MD, Jones D. Lipids in the classification and identification of coryneform bacteria containing peptidoglycan based on 2,4–diaminobutyric acid. Appl Bacteriol 1980; 48:459–470 [Crossref]
     [Google Scholar]
  14. Collins MD, Pirouz T, Goodfellow M, Minnikin DE. Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 1977; 100:221–230 [View Article][PubMed]
     [Google Scholar]
  15. Kroppenstedt RM. Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. J Liq Chromatograph 1982; 5:2359–2387 [Crossref]
     [Google Scholar]
  16. Mesbah M, Premachandran U, Whitman WB. Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 1989; 39:159–167 [Crossref]
     [Google Scholar]
  17. Lau KW, Ren J, Fung MC, Woo PC, Yuen KY et al. Fangia hongkongensis gen. nov., sp. nov., a novel gammaproteobacterium of the order Thiotrichales isolated from coastal seawater of Hong Kong. Int J Syst Evol Microbiol 2007; 57:2665–2669 [View Article][PubMed]
     [Google Scholar]
  18. Sjödin A, Svensson K, Ohrman C, Ahlinder J, Lindgren P et al. Genome characterisation of the genus Francisella reveals insight into similar evolutionary paths in pathogens of mammals and fish. BMC Genomics 2012; 13:268 [View Article][PubMed]
     [Google Scholar]
  19. Beier CL, Horn M, Michel R, Schweikert M, Görtz HD et al. The genus Caedibacter comprises endosymbionts of Paramecium spp. related to the Rickettsiales (Alphaproteobacteria) and to Francisella tularensis (Gammaproteobacteria). Appl Environ Microbiol 2002; 68:6043–6050 [View Article][PubMed]
     [Google Scholar]
  20. Sjöstedt AB. Francisellaceae fam. nov. In Bergey’s Manual of Systematics of Archaea and Bacteria John Wiley & Sons, Inc; 2015 pp. 1–2
     [Google Scholar]
  21. Sjöstedt AB.  Francisella . In Bergey’s Manual of Systematics of Archaea and Bacteria John Wiley & Sons, Inc; 2015 pp. 1–18
     [Google Scholar]
  22. Fryer JL, Lannan CN. Piscirickettsiaceae fam. nov. In Bergey’s Manual of Systematics of Archaea and Bacteria John Wiley & Sons, Inc; 2015 pp. 1
     [Google Scholar]
  23. Fryer JL, Lannan CN.  Piscirickettsia . In Bergey’s Manual of Systematics of Archaea and Bacteria John Wiley & Sons, Inc; 2015 pp. 1–8
     [Google Scholar]
  24. Geiselbrecht AD.  Cycloclasticus . In Bergey’s Manual of Systematics of Archaea and Bacteria John Wiley & Sons, Inc; 2015 pp. 1–6
     [Google Scholar]
  25. Nishihara H.  Hydrogenovibrio . In Bergey’s Manual of Systematics of Archaea and Bacteria John Wiley & Sons, Inc; 2015 pp. 1–4
     [Google Scholar]
  26. Bowman JP.  Methylophaga . In Bergey’s Manual of Systematics of Archaea and Bacteria John Wiley & Sons, Inc; 2015 pp. 1–6
     [Google Scholar]
  27. The Editorial Board  Thioalkalimicrobium . In Bergey’s Manual of Systematics of Archaea and Bacteria John Wiley & Sons, Inc; 2015 pp. 1–2
     [Google Scholar]
  28. Brinkhoff T, Kuever J, Muyzer G, Jannasch HW.  Thiomicrospira . In Bergey’s Manual of Systematics of Archaea and Bacteria John Wiley & Sons, Inc; 2015 pp. 1–10
     [Google Scholar]
  29. Garrity GM, Bell JA, Lilburn T. Thiotrichaceae fam. nov. In Bergey’s Manual of Systematics of Archaea and Bacteria John Wiley & Sons, Inc; 2015 pp. 1
     [Google Scholar]
  30. Unz RF, Head IM.  Thiothrix . In Bergey’s Manual of Systematics of Archaea and Bacteria John Wiley & Sons, Inc; 2015 pp. 1–16
     [Google Scholar]
  31. Strohl WR.  Beggiatoa . In Bergey’s Manual of Systematics of Archaea and Bacteria John Wiley & Sons, Inc; 2015 pp. 1–23
     [Google Scholar]
  32. Tanaka N, Romanenko LA, Iino T, Frolova GM, Mikhailov VV. Cocleimonas flava gen. nov., sp. nov., a gammaproteobacterium isolated from sand snail (Umbonium costatum). Int J Syst Evol Microbiol 2011; 61:412–416 [View Article][PubMed]
     [Google Scholar]
  33. Bland JA, Brock TD.  Leucothrix . In Bergey’s Manual of Systematics of Archaea and Bacteria John Wiley & Sons, Inc; 2015 pp. 1–23
     [Google Scholar]
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Fastidiosibacter lacustris gen. nov., sp. nov., isolated from a lake water sample, and proposal of Fastidiosibacteraceae fam. nov. within the order Thiotrichales
Int J Syst Evol Microbiol 68, 347 (2018); https://doi.org/10.1099/ijsem.0.002510
/content/journal/ijsem/10.1099/ijsem.0.002510
/content/journal/ijsem/10.1099/ijsem.0.002510
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