1887

Abstract

A bacterial strain, 1-14, was isolated from cyanobacterial aggregates in a eutrophic lake, Taihu Lake, China. Cells were observed to be slightly curved, rod-shaped, aerobic and Gram-stain-negative. Optimal growth occurred at pH 7.0 (range: 5.0–9.0), 28 °C (range: 20–32 °C) and 0 % (w/v) NaCl (range: 0–1.0 %) in R2A broth. No growth is observed at 37 °C. The cells were found to be positive for oxidase and catalase activities. The major respiratory quinone was ubiquinone Q-10. The major fatty acids (>10 %) were identified as Cω6/Cω7, C 3-OH and C 2-OH. The major polar lipids were found to consist of phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol and phosphatidylcholine. Within the genus , strain 1-14 was most closely related to TH16 (98.3 % 16S rRNA gene sequence similarity), followed by DSM 11586 (97.8 %) and CC-LY736 (97.0 %). The genomic G+C content of strain 1-14 was 62.2 mol% based on total genome calculations. Genes coding for light-harvesting complexes LHI and LHII, and a photosynthetic reaction centre were detected in the genome. Average nucleotide identities and digital DNA–DNA hybridizations for complete genomes ranged from 76.4 to 83.5 and from 21.5 to 27.4 % between strain 1-14 and strains within the genus . The phenotypic, chemotaxonomic and phylogenetic properties, and genome analysis suggested that strain 1-14 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is 1-14 (=CGMCC 1.12980=LMG 28363).

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/content/journal/ijsem/10.1099/ijsem.0.002526
2018-02-01
2024-03-28
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