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Volume 68, Issue 2

sp. nov., isolated from Pu’er tea Free

Abstract

A novel aerobic, Gram-stain-positive, sporogenous, rod-shaped bacterial strain, 7578-1, was isolated from ripened Pu’er tea. Based on 16S rRNA gene sequence similarity comparisons, strain 7578-1 was grouped into the genus and appeared to be closely related to the type strains LMG 18435 (98.4 %), DSM 14745 (97.6 %), KACC 18426 (97.2 %) and KCTC 13944 (96.7 %). The fatty acid profile containing the major fatty acids, iso-C, anteiso-C and anteiso-C supported the allocation of strain 7578-1 to the genus . The strain had a cell-wall type A1γ peptidoglycan with -diaminopimelic acid as the diagnostic diamino acid. The major menaquinone was MK-7 (95 %). The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, one unidentified phospholipid and one unidentified lipid. The average nucleotide identity values between strain 7578-1 and its most closely related species were 67.8–82.4 % by OrthoANIu analysis. The DNA–DNA relatedness value between strain 7578-1 and the type strains of closely related species were 17–39 %, again indicating that strain 7578-1 represented a novel species in the genus . The DNA GC content of strain 7578-1 was 36.0 mol%. On the basis of the presented polyphasic evidence, strain 7578-1 is considered to represent a novel species of the genus , for which we propose the name sp. nov. The type strain is 7578-1 (=CGMCC 1.15374=KCTC 33845).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Bacillus camelliae sp. nov. , polyphasic analysis and Pu'er tea
© 2018 IUMS
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2018-02-01
2024-04-23
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Bacillus camelliae sp. nov., isolated from Pu’er tea
Int J Syst Evol Microbiol 68, 564 (2018); https://doi.org/10.1099/ijsem.0.002542
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