RT Journal Article SR Electronic(1) A1 Dahal, Ram Hari A1 Kim, JaisooYR 2018 T1 Brevundimonas humi sp. nov., an alphaproteobacterium isolated from forest soil JF International Journal of Systematic and Evolutionary Microbiology, VO 68 IS 3 SP 709 OP 714 DO https://doi.org/10.1099/ijsem.0.002559 PB Microbiology Society, SN 1466-5034, AB During a study of bacterial diversity of soil, a novel strain, CA-15T, was isolated from Kyonggi University forest soil. Cells were aerobic, Gram-stain-negative, motile, non-spore-forming, rod-shaped, oxidase-positive and catalase- negative. Tyrosine was not oxidized but produced red pigmentation on an agar palte. Strain CA-15T hydrolysed Tween 60 and DNA. It grew at 15–35 °C (optimum, 25–30 °C), pH 6.0–10.0 (optimum, 7.0–9.0) and at 1.5 % (w/v) NaCl concentration. Phylogenetic analysis based on its 16S rRNA gene sequence indicated that strain CA-15T formed a lineage within the family Caulobacteraceae of the class Alphaproteobacteria that was distinct from various species of the genus Brevundimonas . Brevundimonas bullata DSM 7126T was the closest member of strain CA-15T on the basis of 16S rRNA gene sequence similarity (98.48 %). Q-10 was only an isoprenoid quinone detected for strain CA-15T. The major polar lipids were 1,2-di-O-acyl-3-O-[d-glucopyranosyl-(1→4)-α d-glucopyranuronosyl]glycerol, 1,2-di-O-acyl-3-O-[α d-glucopyranosyl]-sn-glycerol, 1,2-di-O-acyl-3-O-α d-glucopyranuronosylglycerol, 1,2-diacyl-3-O-[6′-phosphatidyl-α d-glucopyranosyl]glycerol and phosphatidylglycerol. The major cellular fatty acids were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c), C16 : 0, C18 : 1ω7c 11-methyl and C17 : 1ω8c. The DNA G+C content of strain CA-15T was 63.6 mol%. The polyphasic characterization indicated that strain CA-15T represents a novel species in the genus Brevundimonas , for which the name Brevundimonas humi sp. nov. is proposed. The type strain of Brevundimonas humi is CA-15T (=KEMB 9005-528T=KACC 19106T=NBRC 112677T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002559