RT Journal Article SR Electronic(1) A1 Ko, Yongseok A1 Yim, Jeeyeo A1 Hwang, Woon Mo A1 Kang, Keunsoo A1 Ahn, Tae-YoungYR 2018 T1 Roseomonas fluminis sp. nov. isolated from sediment of a shallow stream JF International Journal of Systematic and Evolutionary Microbiology, VO 68 IS 3 SP 782 OP 787 DO https://doi.org/10.1099/ijsem.0.002578 PB Microbiology Society, SN 1466-5034, AB An aerobic, Gram-negative, motile by means of a single polar flagellum, and ovoid-shaped bacterium, designated D3T, was isolated from shallow stream sediments in Sinan-gun, South Korea. Growth occurred at 15–40 °C (optimum 35 °C), at pH 7.0–8.0 (optimum pH 7.0), and at an optimum NaCl concentration of 0.5 % (w/v). The major cellular fatty acids (>7 % of the total) were C16 : 0, C18 : 0 2-OH, summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c) and summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c). The predominant quinone was ubiquinone-10, and the G+C content of the genomic DNA of strain D3T was 73.1 mol%. The major polyamine was spermidine. The major polar lipids of the isolate were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine and phosphatidylglycerol. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain D3T clustered with Roseomonas aquatica TR53T within the genus Roseomonas . The 16S rRNA gene sequence of strain D3T showed the highest sequence similarity to R. aquatica TR53T (95.9 %), followed by Roseomonas rosea 173-96T (95.7 %) and Roseomonas aerilata 5420S-30T (95.0 %). Based on the phenotypic, phylogenetic and chemotaxonomic characterization, strain D3T represents a novel species of the genus Roseomonas , for which the name Roseomonas fluminis sp. nov. is proposed. The type strain is D3T (=KACC 19269T=JCM 31968T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002578