1887

Abstract

A Gram-negative, yellow-pigmented, non-flagellated, gliding, rod-shaped and aerobic bacterium, designated MEBiC 12267, was isolated from green algae of Jeju Island. 16S rRNA gene sequence analysis revealed that the strain MEBiC 12267 was affiliated to the genus of the family and showed the highest similarity to KCTC 42440 (98.5 %). The DNA–DNA relatedness value of strain MEBiC 12267 with KCTC 42440 was 25 %. Growth was observed at 10–37 °C (optimum, 30–33 °C), at pH 6.0–9.5 (optimum, 8.0–8.5) and with 0.5–9.0 % (w/v) NaCl (optimum, 2.5–3.5 %). The predominant cellular fatty acids were iso-C, iso-C G and iso-C 3-OH. The major respiratory quinone was MK-6. Polar lipids included phosphatidylethanolamine, seven unidentified lipids and two unidentified aminolipids. The DNA G+C content was 40.7 mol%. On the basis of the data from the polyphasic taxonomic study, it was concluded that the strain MEBiC 12267 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain of is MEBiC 12267 (=KCCM 43264=JCM 32170).

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2018-03-01
2024-03-19
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References

  1. Garrity GM, Holt JG. The road map to the manual. In Boone DR, Castenholz RW, Garrity GM. (editors) Bergey's Manual of Systematic Bacteriology, 2nd ed. vol. 1 New York: Springer; 2001 pp. 119–166 [Crossref]
    [Google Scholar]
  2. Jooste PJ. The Taxonomy and Significance of Flavobacterium–Cytophaga Strains from Daily Sources PhD thesis University of the Orange Free State, Bloemfontein, South Africa: 1985
    [Google Scholar]
  3. Reichenbach H. The order Cytophagales . In Balows A, Trüper HG, Dworkin M, Harder W, Schleifer KH et al. (editors) The Prokaryotes New York: Springer; 1992 pp. 3631–3679 [Crossref]
    [Google Scholar]
  4. Bernardet J-F, Segers P, Vancanneyt M, Berthe F, Kersters K et al. Cutting a Gordian Knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (Basonym, Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Bacteriol 1996; 46:128–148 [View Article]
    [Google Scholar]
  5. Bernardet JF, Nakagawa Y, Holmes B. Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 2002; 52:1049–1070 [View Article][PubMed]
    [Google Scholar]
  6. Lucena T, Pascual J, Giordano A, Gambacorta A, Garay E et al. Euzebyella saccharophila gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae . Int J Syst Evol Microbiol 2010; 60:2871–2876 [View Article][PubMed]
    [Google Scholar]
  7. Zhang YJ, Zhao JR, Zhang XY, Chen GZ, Zhou MY et al. Euzebyella marina sp. nov., isolated from seawater. Int J Syst Evol Microbiol 2017; 67:920–924 [View Article][PubMed]
    [Google Scholar]
  8. Smibert RM, Krieg NR. Phenotypic characterization. In Gerhardt P, Murray RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994 pp. 607–654
    [Google Scholar]
  9. Komagata K, Suzuki K. Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 1987; 19:161–207 [Crossref]
    [Google Scholar]
  10. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
    [Google Scholar]
  11. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012; 62:716–721 [View Article][PubMed]
    [Google Scholar]
  12. Tamura K, Dudley J, Nei M, Kumar S. MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 2007; 24:1596–1599 [View Article][PubMed]
    [Google Scholar]
  13. Kim YG, Choi DH, Hyun S, Cho BC. Oceanobacillus profundus sp. nov., isolated from a deep-sea sediment core. Int J Syst Evol Microbiol 2007; 57:409–413 [View Article][PubMed]
    [Google Scholar]
  14. Rosselló-Mora R, Amann R. The species concept for prokaryotes. FEMS Microbiol Rev 2001; 25:39–67 [View Article][PubMed]
    [Google Scholar]
  15. Bernardet JF, Nakagawa Y. An introduction to the family Flavobacteriaceae . In Dworkin M, Falkow S, Rosenberg E, Schleifer KH, Stackebrandt E et al. (editors) The Prokaryotes, a Handbook on the Biology of Bacteria, 3rd ed. vol. 7 New York: Springer; 2006 pp. 455–480
    [Google Scholar]
  16. Yi H, Chun J. Unification of the genera Nonlabens, Persicivirga, Sandarakinotalea and Stenothermobacter into a single emended genus, Nonlabens, and description of Nonlabens agnitus sp. nov. Syst Appl Microbiol 2012; 35:150–155 [View Article][PubMed]
    [Google Scholar]
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