RT Journal Article SR Electronic(1) A1 Pal, Mohinder A1 Kumari, Munesh A1 Kiran, Shashi A1 Salwan, Richa A1 Mayilraj, Shanmugam A1 Chhibber, Sanjay A1 Gulati, ArvindYR 2018 T1 Chryseobacterium glaciei sp. nov., isolated from the surface of a glacier in the Indian trans-Himalayas JF International Journal of Systematic and Evolutionary Microbiology, VO 68 IS 3 SP 865 OP 870 DO https://doi.org/10.1099/ijsem.0.002600 PB Microbiology Society, SN 1466-5034, AB A novel bacterial strain, IHBB 10212T, of the genus Chryseobacterium was isolated from a glacier near the Kunzum Pass located in the Lahaul-Spiti in the North-Western Himalayas of India. The cells were Gram-negative, aerobic, non-sporulating, single rods, lacked flagella, and formed yellow to orange pigmented colonies. The strain utilized maltose, trehalose, sucrose, gentibiose, glucose, mannose, fructose, mannitol, arabitol and salicin for growth. Flexirubin-type pigments were produced by strain IHBB 10212T. The 16S rRNA gene sequence analysis showed relatedness of strain IHBB 10212T to Chryseobacterium polytrichastri DSM 26899T (97.43 %), Chryseobacterium greenlandense CIP 110007T (97.29 %) and Chryseobacterium aquaticum KCTC 12483T (96.80 %). Iso-C15 : 0 and summed feature 3 (C16 : 1ω7c/C16 : 1ω6c) constituted the major cellular fatty acids. The polar lipids present were six unidentified aminolipids, one unidentified phospholipid and three unidentified lipids. MK-6 was identified as the major quinone. The DNA G+C content was 34.08  mol%. Digital DNA–DNA hybridization of strain IHBB 10212T with C. polytrichastri , C. greenlandense and C. aquaticum showed values far below the prescribed thresholds of 95 % for average nucleotide identity and 70 % for the Genome-to-Genome Distance Calculator for species delineation. Based on its differences from validly published Chryseobacterium species, strain IHBB 10212T is identified as a new species, for which the proposed name is Chryseobacterium glaciei sp. nov., with IHBB 10212T as the type strain (=MTCC 12457T=JCM 31156T=KACC 19170T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002600