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Volume 68, Issue 3

sp. nov., a betaproteobacterium isolated from stream bank soil Free

Abstract

Two strains of soil bacteria, designated CA-16 and CA-161, were isolated from a sample of stream bank soil near Kyonggi University. Cells were strictly aerobic, Gram-stain-negative, catalase-negative, oxidase-positive, motile, non-spore-forming and rod-shaped. Colonies on tryptone soya agar were brownish cream in colour. Tyrosine, Tween 60 and Tween 40 were hydrolysed. The indole test was positive. Malic acid, lactic acid and valeric acid were assimilated. Phylogenetic analysis based on their 16S rRNA gene sequences revealed that strains CA-16 and CA-161 formed a lineage within the family of the class that were distinct from various species of the genus . Strain CA-16 was most closely related to DSM 1837 (97.86 % sequence similarity), EMB325 (97.72 %), DSM 11588 (96.83 %) and RSG39 (96.71 %). Both strains contained Q-8 as the major isoprenoid quinone. The major polar lipids of the strains were phosphatidylglycerol, phosphatidylethanolamine and diphosphatidylglycerol. The major cellular fatty acids were summed feature 3 (Cω7 and/or Cω6), C 7-11methyl, C, summed feature 8 (Cω7 and/or Cω6) and C. The DNA G+C contents of the strains were 63.9–64.4 mol%. DNA–DNA hybridization similarities between strain CA-16 and other closest members of the genus ranged from 16 % to 24 %. On the basis of phenotypic, genotypic, chemotaxonomic and phylogenetic analyses, strains CA-16 and CA-161 represent a single novel species of the genus , for which the name sp. nov. is proposed. The type strain is CA-16 (=KEMB 9005-529=KACC 19107=NBRC 112689).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Betaproteobacteria , Comamonadaceae , Simplicispira soli sp. nov. , stream bank soil and tyrosine hydrolysis
© 2018 IUMS
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2018-03-01
2024-04-24
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Simplicispira soli sp. nov., a betaproteobacterium isolated from stream bank soil
Int J Syst Evol Microbiol 68, 951 (2018); https://doi.org/10.1099/ijsem.0.002618
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