1887

Abstract

Abacterial strain, designated MMFC1, was isolated from a methanol-fed microbial fuel cell that had been inoculated with sludge obtained from a facility in a chemical plant. The strain grows by fermenting methanol to produce acetate under anaerobic conditions, while homoacetogenic growth is not observed. MMFC1 also grows on pyruvate and lactate but not on sugars and other organic acids. Cells are curved rods and motile, have peritrichous flagella, and form endospores. The genome sequence of strain MMFC1 supports the physiological data. Phylogenetic analysis based on the 16S rRNA gene sequence shows that strain MMFC1 is affiliated with the family , while the closest relative is with milarity of 93.5 %. Major fatty acids are iso-C 3-OH, C 9 and iso-C. On the basis of its physiological, genomic and phylogenetic features, a novel genus and species are proposed to accommodate strain MMFC1, with the name gen. nov., sp. nov. The type strain of is MMFC1 (=JCM 31821 = KCTC 15592).

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2018-04-01
2024-03-28
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References

  1. Asai Y, Miyahara M, Kouzuma A, Watanabe K. Comparative evaluation of wastewater-treatment microbial fuel cells in terms of organics removal, waste-sludge production, and electricity generation. Bioresour Bioprocess 2017; 4:30 [View Article][PubMed]
    [Google Scholar]
  2. Logan BE, Hamelers B, Rozendal R, Schröder U, Keller J et al. Microbial fuel cells: methodology and technology. Environ Sci Technol 2006; 40:5181–5192[PubMed] [Crossref]
    [Google Scholar]
  3. Watanabe K. Recent developments in microbial fuel cell technologies for sustainable bioenergy. J Biosci Bioeng 2008; 106:528–536 [View Article][PubMed]
    [Google Scholar]
  4. Yamamuro A, Kouzuma A, Abe T, Watanabe K. Metagenomic analyses reveal the involvement of syntrophic consortia in methanol/electricity conversion in microbial fuel cells. PLoS One 2014; 9:e98425 [View Article][PubMed]
    [Google Scholar]
  5. Möller B, Oßmer R, Howard BH, Gottschalk G, Hippe H. Sporomusa, a new genus of gram-negative anaerobic bacteria including Sporomusa sphaeroides spec. nov. and Sporomusa ovata spec. nov. Arch Microbiol 1984; 139:388–396 [View Article]
    [Google Scholar]
  6. Kouzuma A, Meng XY, Kimura N, Hashimoto K, Watanabe K. Disruption of the putative cell surface polysaccharide biosynthesis gene SO3177 in Shewanella oneidensis MR-1 enhances adhesion to electrodes and current generation in microbial fuel cells. Appl Environ Microbiol 2010; 76:4151–4157 [View Article][PubMed]
    [Google Scholar]
  7. Watanabe K, Kodama Y, Kaku N. Diversity and abundance of bacteria in an underground oil-storage cavity. BMC Microbiol 2002; 2:23 [View Article][PubMed]
    [Google Scholar]
  8. Watanabe K, Hamamura N, Kaku N. Molecular identification of microbial populations in petroleum-contaminated groundwater. Environ Microbiol 2004; 16:235–242 [Crossref]
    [Google Scholar]
  9. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011; 28:2731–2739 [View Article][PubMed]
    [Google Scholar]
  10. Camacho C, Coulouris G, Avagyan V, Ma N, Papadopoulos J et al. BLAST+: architecture and applications. BMC Bioinformatics 2009; 10:421 [View Article][PubMed]
    [Google Scholar]
  11. Yarza P, Yilmaz P, Pruesse E, Glöckner FO, Ludwig W et al. Uniting the classification of cultured and uncultured bacteria and archaea using 16S rRNA gene sequences. Nat Rev Microbiol 2014; 12:635–645 [View Article][PubMed]
    [Google Scholar]
  12. Beveridge TJ, Popkin TJ, Cole RM. Electron microscopy. In Gerhardt P, Murray RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994 pp. 42–71
    [Google Scholar]
  13. Santo LY, Doi RH. Ultrastructural analysis during germination and outgrowth of Bacillus subtilis spores. J Bacteriol 1974; 120:475–481[PubMed]
    [Google Scholar]
  14. Breznak JA. The genus Sporomusa . In Dworkin MM, Falkow S, Rosenberg E, Schleifer KH, Stackebrandt E et al. (editors) The prokaryotes-Bacteria: Firmicutes, Cyanobacteria New York: Springer; 2006 pp. 991–1001
    [Google Scholar]
  15. Smibert RM, Krieg NR. Phenotypic characterization. In Gerhardt P, Murray RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994 pp. 607–655
    [Google Scholar]
  16. Balk M, Mehboob F, van Gelder AH, Rijpstra WI, Damsté JS et al. (Per)chlorate reduction by an acetogenic bacterium, Sporomusa sp., isolated from an underground gas storage. Appl Microbiol Biotechnol 2010; 88:595–603 [View Article][PubMed]
    [Google Scholar]
  17. Wilson K. Preparation of genomic DNA from bacteria. Curr Protoc Mol Biol 2001; Chapter 2:Unit 2.4 [View Article][PubMed]
    [Google Scholar]
  18. Seemann T. Prokka: rapid prokaryotic genome annotation. Bioinformatics 2014; 30:2068–2069 [View Article][PubMed]
    [Google Scholar]
  19. Tatusov RL, Koonin EV, Lipman DJ. A genomic perspective on protein families. Science 1997; 278:631–637 [View Article][PubMed]
    [Google Scholar]
  20. Browne HP, Forster SC, Anonye BO, Kumar N, Neville BA et al. Culturing of 'unculturable' human microbiota reveals novel taxa and extensive sporulation. Nature 2016; 533:543–546 [View Article][PubMed]
    [Google Scholar]
  21. Ollivier B, Cordruwisch R, Lombardo A, Garcia JL. Isolation and characterization of Sporomusa acidovorans sp. nov., a methylotrophic homoacetogenic bacterium. Arch Microbiol 1985; 142:307–310 [View Article]
    [Google Scholar]
  22. Hermann M, Popoff M-R, Sebald M. Sporomusa paucivorans sp. nov., a methylotrophic bacterium that forms acetic acid from hydrogen and carbon dioxide. Int J Syst Bacteriol 1987; 37:93–101 [View Article]
    [Google Scholar]
  23. Sass H, Overmann J, Rütters H, Babenzien HD, Cypionka H. Desulfosporomusa polytropa gen. nov., sp. nov., a novel sulfate-reducing bacterium from sediments of an oligotrophic lake. Arch Microbiol 2004; 182:204–211 [View Article][PubMed]
    [Google Scholar]
  24. Poehlein A, Gottschalk G, Daniel R. First insights into the genome of the Gram-negative, endospore-forming organism Sporomusa ovata strain H1 DSM 2662. Genome Announc 2013; 1:e00734-13 [View Article][PubMed]
    [Google Scholar]
  25. Villamizar GA, Daniel R, Poehlein A. First insights into the genome sequence of the strictly anaerobic homoacetogenic Sporomusa sphaeroides strain E (DSM 2875). Genome Announc 2017; 5:e00037-17 [View Article][PubMed]
    [Google Scholar]
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