1887

Abstract

A Gram-stain-negative, facultatively anaerobic strain, designated FJ201301, was isolated from aquaculture water collected from Fujian province, China. Phylogenetic analysis of 16S rRNA gene sequences indicated that strain FJ201301 belonged to the genus , formed a distinct cluster with ATCC 35912 and shared the highest similarity with CGMCC 1.12427. A 15 bp insertion found in the 16S rRNA gene was a significant marker that distinguished strain FJ201301 from several phylogenetic neighbours (e.g. ). Multilocus sequence analysis of eight genes (, , , , , , and ; concatenated 4135 bp sequence) showed that, forming a long and independent phylogenetic branch, strain FJ201301 clustered with ATCC 35912, KCTC 32233 and CIP 69.14 clearly separated from CGMCC 1.12427. Furthermore, the highest DNA–DNA hybridization and average nucleotide identity values between strain FJ201301 and the closest related species were 26.3 and 83.1 % with ATCC 35912, less than the proposed cutoff levels for species delineation, i.e. 70 and 95 %, respectively. Biochemical, sequence and genomic analysis suggested the designation of strain FJ201301 representing a novel species of the genus , for which the name sp. nov. is proposed. The type strain is FJ201301 (=DSM 104687=CGMCC 1.16099).

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2018-04-01
2024-03-28
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