%0 Journal Article %A Pérez-Cataluña, Alba %A Salas-Massó, Nuria %A Figueras, María José %T Arcobacter canalis sp. nov., isolated from a water canal contaminated with urban sewage %D 2018 %J International Journal of Systematic and Evolutionary Microbiology, %V 68 %N 4 %P 1258-1264 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.002662 %K isDDH %K A. canalis %K 16S rRNA %K MLPA %K shellfish %K ANI %K Arcobacter %I Microbiology Society, %X Four bacterial strains recovered from shellfish (n=3) and from the water (n=1) of a canal contaminated with urban sewage were recognized as belonging to a novel species of the genus Arcobacter (represented by strain F138-33T) by using a polyphasic characterization. All the new isolates required 2 % NaCl to grow. Phylogenetic analyses based on 16S rRNA gene sequences indicated that all strains clustered together, with the most closely related species being Arcobacter marinus and Arcobacter molluscorum . However, phylogenetic analyses using the concatenated sequences of housekeeping genes (atpA, gyrB, hsp60, gyrA and rpoB) showed that all the novel strains formed a distinct lineage within the genus Arcobacter . Results of in silico DNA–DNA hybridization and the average nucleotide identity between the genome of strain F138-33T and those of the closely related species A. marinus and other relatively closely related species such as A. molluscorum and Arcobacter halophilus were all below 70 and 96 %, respectively. All the above results, together with the 15 physiological and biochemical tests that could distinguish the newly isolated strains from the closely related species, confirmed that these strains represent a novel species for which the name Arcobacter canalis sp. nov. is proposed, with the type strain F138-33T (=CECT 8984T=LMG 29148T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002662