@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.002667, author = "Chen, Wen-Ming and Xie, Pei-Bei and Hsu, Ming-yuan and Sheu, Shih-Yi", title = "Parvibium lacunae gen. nov., sp. nov., a new member of the family Alcaligenaceae isolated from a freshwater pond", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2018", volume = "68", number = "4", pages = "1291-1299", doi = "https://doi.org/10.1099/ijsem.0.002667", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002667", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Burkholderiales", keywords = "Betaproteobacteria", keywords = "Proteobacteria", keywords = "freshwater pond", keywords = "new taxa", keywords = "Parvibium lacunae", abstract = "A bacterial strain designated KMB9T was isolated from a freshwater pond in Taiwan and characterized using a polyphasic taxonomy approach. Cells of strain KMB9T were Gram-stain-negative, aerobic, poly-β-hydroxybutyrate-accumulating, motile by means of a monopolar flagellum, non-spore-forming and rods surrounded by a thick capsule and forming white-coloured colonies. Growth occurred at 20–40 °C (optimum, 25–37 °C), at pH 6.5–7.5 (optimum, pH 7.0) and with 0–0.5 % NaCl (optimum, 0 %). Phylogenetic analyses based on 16S rRNA gene and four housekeeping gene sequences (recA, rpoA, rpoB and atpD) showed that strain KMB9T forms a distinct phyletic line within the family Alcaligenaceae , and the levels of 16S rRNA gene sequence similarity to its closest relatives with validly published names were less than 93.3 %. The predominant fatty acids were summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), C16 : 0 and C18 : 1ω7c. The major isoprenoid quinone was Q-8. The major polyamine was putrescine. The polar lipid profile revealed the presence of phosphatidylethanolamine, phosphatidylglycerol and several uncharacterized aminophospholipids, aminolipids, phospholipids and lipids. The genomic DNA G+C content of strain KMB9T was 54.5 mol%. On the basis of the genotypic and phenotypic data, strain KMB9T represents a novel species of a new genus in the family Alcaligenaceae , for which the name Parvibium lacunae gen. nov., sp. nov. is proposed. The type strain is KMB9T (=BCRC 81053T=LMG 30055T=KCTC 52814T).", }