@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.002674, author = "Schumann, Peter and Zhang, De-Chao and França, Luís and Margesin, Rosa", title = "Marmoricola silvestris sp. nov., a novel actinobacterium isolated from alpine forest soil", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2018", volume = "68", number = "4", pages = "1313-1318", doi = "https://doi.org/10.1099/ijsem.0.002674", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002674", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "alpine", keywords = "Marmoricola", keywords = "forest soil", keywords = "cold-adapted", keywords = "actinobacterium", abstract = "A Gram-stain-positive, flagellated, catalase- and cytochrome c oxidase-positive bacterial strain, designated S20-100T, was isolated from alpine forest soil. Growth occurred at a temperature range of 0–30 °C, at pH 6–9 and in the presence of 0–1 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain S20-100T was related to the genus Marmoricola and had the highest 16S rRNA gene sequence similarity to Marmoricola ginsengisoli Gsoil 097T (98.4 %) and Marmoricola solisilvae KIS18-7T (98.3 %). The cell-wall peptidoglycan of strain S20-100T contained ll-diaminopimelic acid (ll-Dpm) as the diagnostic diamino acid and was of the type A3γ ll-Dpm – Gly. The strain contained MK-8(H4) as the predominant isoprenoid quinone and diphosphatidylglycerol, phosphatidylglycerol, four unidentified phospholipids and three unidentified lipids in lower amounts. The major cellular fatty acids (>10 %) were iso-C16 : 0, C17 : 1ω6c and C18 : 1ω9c. The genomic DNA G+C content was 66.2 mol%. Combined data of phylogenetic, phenotypic and chemotaxonomic analyses demonstrated that strain S20-100T represents a novel species of the genus Marmoricola , for which the name Marmoricola silvestris sp. nov. is proposed. The type strain is S20-100T (=DSM 104694T=LMG 30008T).", }