@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.002682, author = "Penkhrue, Watsana and Sujarit, Kanaporn and Kudo, Takuji and Ohkuma, Moriya and Masaki, Kazuo and Aizawa, Tomoyasu and Pathom-aree, Wasu and Khanongnuch, Chartchai and Lumyong, Saisamorn", title = "Amycolatopsis oliviviridis sp. nov., a novel polylactic acid-bioplastic-degrading actinomycete isolated from paddy soil", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2018", volume = "68", number = "5", pages = "1448-1454", doi = "https://doi.org/10.1099/ijsem.0.002682", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002682", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "actinobacteria", keywords = "Amycolatopsis oliviviridis sp. nov", keywords = "bioplastic degradation", keywords = "polyphasic taxonomy", keywords = "16S rRNA", abstract = "A novel bioplastic-degrading actinomycete, strain SCM_MK2-4T, was isolated from paddy soil in Thailand. The 16S rRNA gene sequence showed that strain SCM_MK2-4T belonged to the genus Amycolatopsis , with the highest sequence similarity to Amycolatopsis azurea JCM 3275T (99.4 %), and was phylogenetically clustered with this strain along with Amycolatopsis lurida JCM 3141T (99.3 %), A. japonica DSM 44213T (99.2 %), A. decaplanina DSM 44594T (99.0 %), A. roodepoortensis M29T (98.9 %), A. keratiniphila subsp. nogabecina DSM 44586T (98.8 %), A. keratiniphila subsp. keratiniphila DSM 44409T (98.5 %), A. orientalis DSM 40040T (98.4 %) and A. regifaucium GY080T (98.3 %). A combination of DNA–DNA hybridization results ranging from 42.8±3.2 to 66.2±1.4 % with the type strains of A. azurea and A. lurida and some different phenotypic characteristics indicated that the strain could be distinguished from its closest phylogenetic neighbours. Whole-cell hydrolysates of the strain were shown to contain meso-diaminopimelic acid, arabinose, galactose, glucose, ribose, mannose, rhamnose and xylose. The predominant menaquinone was MK-9(H4). The major cellular fatty acid profile consisted of iso-C15 : 0, iso-C16 : 0, summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2OH) and C16 : 0. The polar lipid composition of the strain consisted of phosphatidyl-N-methylethylethanolamine, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, phosphatidylglycerol, aminophospholipids, an unidentified phospholipid and two unidentified glycolipids. The G+C content of the genomic DNA was 68.2 mol%. On the basis of phylogenetic analyses, DNA–DNA hybridization experimentation and the phenotypic characteristics, it was concluded that strain SCM_MK2-4T represents a novel species of the genus Amycolatopsis , for which the name Amycolatopsis oliviviridis sp. nov. is proposed. The type strain is SCM_MK2-4T (=TBRC 7186T=JCM 32134T).", }