1887

Abstract

Two strains of Gram-stain-negative, facultatively anaerobic short-rod bacteria were recovered from two different food samples in Ma’anshan city, Anhui province, China in 2008. The bacteria were characterized in a polyphasic taxonomic study that included phenotypic, phylogenetic and genotypic methodologies. Phylogenetic analysis of the 16S rRNA gene demonstrated that the two strains belonged to the genus and were most similar to ATCC 29905 with a score of 99.7 %. Phylogenetic analysis of the gene placed the two strains into a cluster with a distinctly interspecies phylogenetic branch that was clearly separated from six type strains of the genus , with the most closely related species being ATCC 29906. genomic comparisons, including DNA–DNA hybridization (DDH) and average nucleotide identity (ANI) analysis showed that the representative strain, 08MAS0041, and all six species share less than 70 % DDH and have a 95 % ANI cutoff level, supporting the designation of the two strains as a novel species of the genus . The predominant cellular fatty acids of strain 08MAS0041 were C (24.8 %), Cω7/ω6 (16.5 %), Cω6/C 7 (14.5 %), C cyclo (12.6 %) and Ciso I/C 3-OH (10.6 %). The analysis of biochemical, phylogenetic and genomic data confirmed that the two strains were clearly different from all recognized species of the genus and represent a novel species, for which the name sp. nov. is proposed. The type strain is 08MAS0041 (=DSM 104685=CGMCC 1.15939).

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2018-04-01
2024-03-29
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