@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.002689, author = "Dai, Hang and Wang, Yonglu and Fang, Yujie and Huang, Zhenzhou and Kan, Biao and Wang, Duochun", title = "Proteus alimentorum sp. nov., isolated from pork and lobster in Ma’anshan city, China", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2018", volume = "68", number = "4", pages = "1390-1395", doi = "https://doi.org/10.1099/ijsem.0.002689", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002689", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "16S rRNA gene", keywords = "average nucleotide identity", keywords = "rpoB", keywords = "Proteus", keywords = "in silico DDH", abstract = "Two strains of Gram-stain-negative, facultatively anaerobic short-rod bacteria were recovered from two different food samples in Ma’anshan city, Anhui province, China in 2008. The bacteria were characterized in a polyphasic taxonomic study that included phenotypic, phylogenetic and genotypic methodologies. Phylogenetic analysis of the 16S rRNA gene demonstrated that the two strains belonged to the genus Proteus and were most similar to Proteus vulgaris ATCC 29905T with a score of 99.7 %. Phylogenetic analysis of the rpoB gene placed the two strains into a cluster with a distinctly interspecies phylogenetic branch that was clearly separated from six type strains of the genus Proteus , with the most closely related species being Proteus mirabilis ATCC 29906T. In silico genomic comparisons, including in silico DNA–DNA hybridization (isDDH) and average nucleotide identity (ANI) analysis showed that the representative strain, 08MAS0041T, and all six Proteus species share less than 70 % isDDH and have a 95 % ANI cutoff level, supporting the designation of the two strains as a novel species of the genus Proteus . The predominant cellular fatty acids of strain 08MAS0041T were C16 : 0 (24.8 %), C16 : 1ω7c/16 : 1ω6c (16.5 %), C18 : 1ω6c/C18 : 1 ω7c (14.5 %), C17 : 0 cyclo (12.6 %) and C16 : 1iso I/C14 : 0 3-OH (10.6 %). The analysis of biochemical, phylogenetic and genomic data confirmed that the two strains were clearly different from all recognized species of the genus Proteus and represent a novel Proteus species, for which the name Proteus alimentorum sp. nov. is proposed. The type strain is 08MAS0041T (=DSM 104685T=CGMCC 1.15939T).", }