@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.002702, author = "Kämpfer, Peter and Busse, Hans-Jürgen and Glaeser, Stefanie P.", title = "Novosphingobium lubricantis sp. nov., isolated from a coolant lubricant emulsion", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2018", volume = "68", number = "5", pages = "1560-1564", doi = "https://doi.org/10.1099/ijsem.0.002702", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002702", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "coolant lubricant", keywords = "taxonomy", keywords = "Novosphingobium", keywords = "lubricantis", abstract = "A yellow-pigmented, Gram-stain-negative, rod-shaped, non-spore-forming bacterium (strain KSS165-70T) was isolated from a coolant lubricant emulsion. The 16S rRNA gene sequence analysis of strain KSS165-70T showed high sequence similarity to the type strains of Novosphingobium subterraneum (98.1 %), Novosphingobium lentum (97.9 %) and Novosphingobium taihuense (97.8 %). Sequence similarities to type strains of all other Novosphingobium species were below 97.5 %. Ubiquinone Q-10 was detected as the major respiratory quinone. The predominant fatty acid C18 : 1ω7c and the typical 2-hydroxy fatty acid C14 : 0 2-OH were detected. The polar lipid profile contained the major lipids diphosphatidylglycerol, phosphatedylethanolamine, sphingoglycolipid, phosphatidylcholine and two unidentified phospholipids. The polyamine pattern contained the major compound spermidine. Characterization by 16S rRNA gene sequence analysis, physiological parameters, pigment analysis, and ubiquinone, polar lipid and fatty acid composition revealed that strain KSS165-70T represents a new species of the genus Novosphingobium . For this reason, we propose the name Novosphingobium lubricantis sp. nov. with the type strain KSS165-70T (=CIP 111490T=CCM 8814T).", }