1887

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Volume 68, Issue 5

sp. nov., an endophytic actinomycete isolated from the roots of (L.) Gaertn. Free

Abstract

A novel actinomycete strain, designated GKU 128, isolated from the roots of an Indian oak tree [ (L.) Gaertn.] at Khao Khitchakut district, Chantaburi province, Thailand, was characterized by using a polyphasic approach. The strain formed a branched substrate and aerial mycelia which differentiated into straight to flexuous chains of smooth-ornamented spores. Analysis of the cell wall revealed the presence of -diaminopimelic acid and -acetylmuramic acid in the peptidoglycan. The whole-cell sugars were glucose, madurose, mannose, rhamnose and ribose. Mycolic acids were absent. The major phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositolmannoside. The predominant menaquinones were MK-9(H), MK-9(H), MK-9(H) and MK-9(H). The major fatty acids were C, Cω9 and 10-methyl C (tuberculostearic acid). The genomic DNA G+C content was 70.5 mol%. Based on 16S rRNA gene sequence analysis, strain GKU 128 was closely related to the type strains of NBRC 15918 (99.2 % sequence similarity) and JCM 9371 (98.7 %). The levels of DNA–DNA relatedness between strain GKU 128 and the closely related type species were less than 19 %. On the basis of phenotypic and genotypic characteristics, strain GKU 128 could be distinguished from its closely related type strains and represents a novel species of the genus , for which the name sp. nov. (=TBRC 7225=NBRC 113074) is proposed.

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Keyword(s): Actinomadura barringtoniae sp. nov. , endophytic actinomycete , Indian oak tree and new species
© 2018 IUMS
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2018-05-01
2024-04-24
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Actinomadura barringtoniae sp. nov., an endophytic actinomycete isolated from the roots of Barringtonia acutangula (L.) Gaertn.
Int J Syst Evol Microbiol 68, 1584 (2018); https://doi.org/10.1099/ijsem.0.002714
/content/journal/ijsem/10.1099/ijsem.0.002714
/content/journal/ijsem/10.1099/ijsem.0.002714
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