@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.002715, author = "Cole, Jessica K. and Morton, Beau R. and Cardamone, Hayley C. and Lake, Hannah R. R. and Dohnalkova, Alice C. and Kim, Young-Mo and Kyle, Jennifer E. and Maezato, Yukari and Dana, Karl L. and Metz, Thomas O. and Romine, Margaret F. and Nelson, William C. and Lindemann, Stephen R.", title = "Salinivirga fredricksonii gen. nov., sp. nov., a heterotrophic halophile isolated from a photosynthetic mat, a member of a novel lineage (Salinarimonadaceae fam. nov.) within the order Rhizobiales, and reclassification of the genus Salinarimonas Liu et al. 2010 into Salinarimonadaceae", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2018", volume = "68", number = "5", pages = "1591-1598", doi = "https://doi.org/10.1099/ijsem.0.002715", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002715", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "epsophile", keywords = "hypersaline", keywords = "meromictic", keywords = "epsomitic lake", keywords = "halophile", abstract = "A halophilic bacterial strain, HL-109T, was isolated from the unicyanobacterial consortium UCC-O, which was obtained from the photosynthetic mat of Hot Lake (Washington, USA). A polyphasic approach using phenotypic, genotypic and chemotaxonomic data was used to classify the strain within the order Rhizobiales . The organism stained Gram-negative and was a moderate thermophile with a growth optimum of 45 °C. It was obligately aerobic, heterotrophic and halophilic, growing in both NaCl and MgSO4 brines. The novel isolate had a polymorphic cellular morphology of short rods with occasional branching, and cells were monotrichous. The major fatty acids detected were C18 : 1, C18 : 0, C16 : 0 and C18 : cyc. Phylogenetic analysis of the 16S rRNA gene placed the strain in the order Rhizobiales and it shared 94 % identity with the type strain of its nearest relative, Salinarimonas ramus . Morphological, chemotaxonomic and phylogenetic results did not affiliate the novel organism with any of the families in the Rhizobiales ; therefore, HL-109T is representative of a new lineage, for which the name Salinivirga fredricksonii gen. nov., sp. nov. is proposed, with the type strain HL-109T (=JCM 31876T=DSM 102886T). In addition, examination of the phylogenetics of strain HL-109T and its nearest relatives, Salinarimonas ramus and Salinarimonas rosea , demonstrates that these halophiles form a clade distinct from the described families of the Rhizobiales . We further propose the establishment of a new family, Salinarimonadaceae fam. nov., to accommodate the genera Salinivirga and Salinarimonas (the type genus of the family).", }