1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 68, Issue 6

sp. nov., an orange-pigmented bacterium isolated from cyanobacterial aggregates in a eutrophic lake Free

Abstract

A bacterial strain, designated TH167, was isolated from cyanobacterial aggregates in eutrophic Lake Taihu in China. Cells were observed to be slightly curved rod-shaped, motile by gliding, aerobic, Gram-stain-negative, proteorhodopsin-containing. Optimal growth was obtained at pH 7.0 (range: 6.0–9.0), 28 °C (range: 4–32 °C) and 0 % (w/v) NaCl (range: 0–2.0 %) in Reasoner’s 2A broth. No growth was observed at 37 °C. The cells were found to be positive for catalase and oxidase activities. The major fatty acids (>10 %) were identified as iso-C, iso-C G and anteiso-C. The major polar lipids of the isolate comprised phosphatidylethanolamine, one unidentified phospholipid and two unidentified aminolipids. The major respiratory quinone was menaquinone-6. The genomic G+C content of strain TH167 was 40.4 mol% based on total genome calculations. Based on similarities of 16S rRNA gene sequences, strain TH167 was affiliated with the genus , exhibiting the highest sequence similarities to SA31 (94.16 %), hg (94.09 %) and NP180 (93.95 %). The phenotypic, chemotaxonomic and phylogenetic properties, and genome analysis suggested that strain TH167 represented a novel species within the genus , for which the name sp. nov. is proposed. The type strain is TH167 (=CGMCC 1.15805=LMG 29719).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): cyanobacterial aggregates , eutrophic lake and Flavobacterium aurantiibacter sp. nov.
© 2018 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002741
2018-06-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/68/6/1839.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002741&mimeType=html&fmt=ahah

References

  1. Bergey DH, Harrison FC, Breed RS, Hammer BW, Huntoon FM et al. (editors) Genus II. Flavobacterium gen. nov. In Bergey’s Manual of Determinative Bacteriology Baltimore, MD: Williams & Wilkins; 1923 pp. 97–117
     [Google Scholar]
  2. Bernardet J-F, Segers P, Vancanneyt M, Berthe F, Kersters K et al. Cutting a Gordian Knot: Emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (Basonym, Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Bacteriol 1996; 46:128–148 [View Article]
     [Google Scholar]
  3. Dong K, Chen F, Du Y, Wang G. Flavobacterium enshiense sp. nov., isolated from soil, and emended descriptions of the genus Flavobacterium and Flavobacterium cauense, Flavobacterium saliperosum and Flavobacterium suncheonense. Int J Syst Evol Microbiol 2013; 63:886–892 [View Article][PubMed]
     [Google Scholar]
  4. Kang JY, Chun J, Jahng KY. Flavobacterium aciduliphilum sp. nov., isolated from freshwater, and emended description of the genus Flavobacterium. Int J Syst Evol Microbiol 2013; 63:1633–1638 [View Article][PubMed]
     [Google Scholar]
  5. Bernardet JF, Bowman JP. Genus I. Flavobacterium Bergey et al. 1923. In Whitman W. (editor) Bergey’s Manual of Systematic Bacteriology, 2nd ed. Baltimore, USA: The Williams & Wilkins Co.; 2011 pp. 112–154
     [Google Scholar]
  6. Hu G, Zhang J, Yang G, Li YY, Guan YT et al. Flavobacterium yanchengense sp. nov., isolated from soil. Int J Syst Evol Microbiol 2013; 63:2848–2852 [View Article][PubMed]
     [Google Scholar]
  7. Hwang WM, Kim D, Kang K, Ahn TY. Flavobacterium eburneum sp. nov., isolated from reclaimed saline land soil. Int J Syst Evol Microbiol 2017; 67:55–59 [View Article][PubMed]
     [Google Scholar]
  8. Li A, Liu H, Sun B, Zhou Y, Xin Y. Flavobacterium lacus sp. nov., isolated from a high-altitude lake, and emended description of Flavobacterium filum. Int J Syst Evol Microbiol 2014; 64:933–939 [View Article][PubMed]
     [Google Scholar]
  9. Cai H, Zeng Y, Wang Y, Cui H, Jiang H. Flavobacterium cyanobacteriorum sp. nov., isolated from cyanobacterial aggregates in a eutrophic lake. Int J Syst Evol Microbiol 2018 [View Article][PubMed]
     [Google Scholar]
  10. Cai H, Jiang H, Krumholz LR, Yang Z. Bacterial community composition of size-fractioned aggregates within the phycosphere of cyanobacterial blooms in a eutrophic freshwater lake. PLoS One 2014; 9:e102879 [View Article][PubMed]
     [Google Scholar]
  11. Cai HY, Yan ZS, Wang AJ, Krumholz LR, Jiang HL. Analysis of the attached microbial community on mucilaginous cyanobacterial aggregates in the eutrophic Lake Taihu reveals the importance of Planctomycetes. Microb Ecol 2013; 66:73–83 [View Article][PubMed]
     [Google Scholar]
  12. Gómez-Consarnau L, González JM, Coll-Lladó M, Gourdon P, Pascher T et al. Light stimulates growth of proteorhodopsin-containing marine Flavobacteria. Nature 2007; 445:210–213 [View Article][PubMed]
     [Google Scholar]
  13. Yoshizawa S, Kawanabe A, Ito H, Kandori H, Kogure K. Diversity and functional analysis of proteorhodopsin in marine Flavobacteria. Environ Microbiol 2012; 14:1240–1248 [View Article][PubMed]
     [Google Scholar]
  14. Yoshizawa S, Kumagai Y, Kim H, Ogura Y, Hayashi T et al. Functional characterization of flavobacteria rhodopsins reveals a unique class of light-driven chloride pump in bacteria. Proc Natl Acad Sci USA 2014; 111:6732–6737 [View Article][PubMed]
     [Google Scholar]
  15. Cai H, Wang K, Huang S, Jiao N, Chen F. Distinct patterns of picocyanobacterial communities in winter and summer in the Chesapeake Bay. Appl Environ Microbiol 2010; 76:2955–2960 [View Article][PubMed]
     [Google Scholar]
  16. Bolger AM, Lohse M, Usadel B. Trimmomatic: a flexible trimmer for Illumina sequence data. Bioinformatics 2014; 30:2114–2120 [View Article][PubMed]
     [Google Scholar]
  17. Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M et al. SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol 2012; 19:455–477 [View Article][PubMed]
     [Google Scholar]
  18. Overbeek R, Olson R, Pusch GD, Olsen GJ, Davis JJ et al. The SEED and the rapid annotation of microbial genomes using subsystems technology (RAST). Nucleic Acids Res 2014; 42:D206–D214 [View Article][PubMed]
     [Google Scholar]
  19. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article][PubMed]
     [Google Scholar]
  20. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997; 25:4876–4882 [View Article][PubMed]
     [Google Scholar]
  21. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article][PubMed]
     [Google Scholar]
  22. Auch AF, von Jan M, Klenk HP, Göker M. Digital DNA–DNA hybridization for microbial species delineation by means of genome-to-genome sequence comparison. Stand Genomic Sci 2010; 2:117–134 [View Article][PubMed]
     [Google Scholar]
  23. Meier-Kolthoff JP, Auch AF, Klenk HP, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013; 14:60 [View Article][PubMed]
     [Google Scholar]
  24. Dong X, Cai M. Identification Manual of Common Bacteria Beijing: Science Press; 2001
     [Google Scholar]
  25. Bernardet JF, Nakagawa Y, Holmes B. Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 2002; 52:1049–1070 [View Article][PubMed]
     [Google Scholar]
  26. Paisley R. MIS Whole Cell Fatty Acid Analysis by Gas Chromatography Training Manual Newark, DE: MIDI; 1996
     [Google Scholar]
  27. Bernardet JF. Family I. Flavobacteriaceae Reichenbach 1992. In Whitman W. (editor) Bergey’s Manual of Systematic Bacteriology, 2nd ed. vol. 4 Baltimore: The Williams & Wilkins Co; 2011 pp. 106–111
     [Google Scholar]
  28. Kates M. Techniques of Lipidology, 2nd ed. Amsterdam: Elsevier; 1986 pp. 106–107
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.002741
Loading
Flavobacterium aurantiibacter sp. nov., an orange-pigmented bacterium isolated from cyanobacterial aggregates in a eutrophic lake
Int J Syst Evol Microbiol 68, 1839 (2018); https://doi.org/10.1099/ijsem.0.002741
/content/journal/ijsem/10.1099/ijsem.0.002741
/content/journal/ijsem/10.1099/ijsem.0.002741
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error