RT Journal Article SR Electronic(1) A1 Castro, David J. A1 Cerezo, Isabel A1 Sampedro, Inmaculada A1 Martínez-Checa, FernandoYR 2018 T1 Roseovarius ramblicola sp. nov., a moderately halophilic bacterium isolated from saline soil in Spain JF International Journal of Systematic and Evolutionary Microbiology, VO 68 IS 6 SP 1851 OP 1856 DO https://doi.org/10.1099/ijsem.0.002744 PB Microbiology Society, SN 1466-5034, AB Strain D15T was isolated from a soil sample taken from Rambla Salada (Murcia), south-eastern Spain, by using the dilution-to-extinction method. The strain, a Gram-stain-negative aerobic bacteria, is non-motile, ovoid- or rod-shaped, catalase- and oxidase-positive, and grows at NaCl concentrations within the range 0.5–10 % (w/v) [optimum 3 % (w/v)], at 5–30 °C (optimum 28 °C) and at pH 6–9 (optimum pH 7.0). The 16S rRNA gene sequence indicates that it belongs to the genus Roseovarius in the class Alphaproteobacteria . Its closest relatives are Roseovarius tolerans EL-172T and Roseovarius azorensis SSW084T, to which the strain shows 16S rRNA gene-sequence similarity values of 96.1 and 95.3 %, respectively. The DNA G+C content is 63 mol%. The major fatty acids (>5 % of the total fatty acids) of strain D15T are C18 : 1ω7c, C16 : 0 and C12 : 0. The only detected isoprenoid quinone of strain D15T is ubiquinone 10 (Q-10). The polar lipid profile contains phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, aminolipid and three polar lipids. Based on the phylogenetic, genotypic, phenotypic and chemotaxonomic data, the strain represents a novel species of the genus Roseovarius , for which the name Roseovarius ramblicola sp. nov. is proposed. Strain D15T (=CECT 9424=LMG 30322) is the type strain., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002744