1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 68, Issue 6

sp. nov., isolated from chickpea ( L.) in China Free

Abstract

Three chickpea rhizobial strains (WYCCWR 10195=S1-3-7, WYCCWR 10198=S1-4-3 and WYCCWR 10200=S1-5-1) isolated from Northwest China formed a group affiliated to based on 16S rRNA gene sequence comparison. To clarify their species status, multilocus sequence analysis and average nucleotide identity (ANI) values of whole genome sequences between the novel group and the type strains of the related species were further performed. Similarities of 95.7–96.6 % in the concatenated sequences of and 91.9–93.1 % of ANI values to the closest-related species , and demonstrated the novel group a unique genospecies. The most abundant fatty acid in cells of WYCCWR 10195 were C cyclo 8 (51.4 %), followed by C 7 11-methyl (9.5 %) and C (9.3 %). Its genome size was 6.37 Mbp, comprising 6633 predicted genes with a DNA G+C content of 61.9 mol%. The similarities of 99.0–99.8 % for the gene and 98.3–99.44 % for the gene to those of the chickpea rhizobial species and nodulation with L. confirmed the strains of the new genospecies as symbiovar ciceri. The weak utilization of most of the tested sugars/organic acids and non-utilization of (+)-rhamnose, -cysteine and -glycine as sole carbon source, tolerance to 1 % (w/v) NaCl, resistance to 5 µg ml chloromycetin and non-hydrolysis of -tyrosine distinguished the novel group from the related species and supported this group as a novel species, for which the name sp. nov. is proposed, with WYCCWR 10195 (=S1-3-7=HAMBI 3692=LMG 30254) as the type strain.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): chickpea , Cicer arietinum , Mesorhizobium wenxiniae sp. nov. and rhizobia
© 2018 IUMS
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2018-06-01
2024-04-19
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Mesorhizobium wenxiniae sp. nov., isolated from chickpea (Cicer arietinum L.) in China
Int J Syst Evol Microbiol 68, 1930 (2018); https://doi.org/10.1099/ijsem.0.002770
/content/journal/ijsem/10.1099/ijsem.0.002770
/content/journal/ijsem/10.1099/ijsem.0.002770
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