1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 68, Issue 7

gen. nov., sp. nov., isolated from mangrove forest sediment Free

Abstract

A Gram-strain-negative, coccoid bacterium, lacking bacteriochlorophyll, designated strain T1lg56, was isolated from a sediment sample collected from Ximen island mangrove forest, Zhejiang province, China. Cells were halotolerant, and catalase- and oxidase-positive. Growth was observed at 18–42 °C (optimum, 35 °C), at pH 6.0–9.5 (optimum, pH 6.5) and in the presence of 0–15 % (w/v) NaCl (optimum, 2–5 %). The major cellular fatty acids were Cω7 and C. The polar lipid profile of strain T1lg56 consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylmonomethylethanolamine, two unidentified phospholipids and five unidentified lipids. Ubiquinone-10 was the predominant respiratory quinone. The assimilation of the substrates in the API 20NE kit was positive in strain T1lg56. The DNA G+C content of strain T1lg56 was 67.2 mol%. 16S rRNA gene sequence analysis indicated that strain T1lg56 was a member of family and was closely related to KMM 9010, with 95.7 % similarity to the type strain. Phylogenetic analysis showed that strain T1lg56 formed a separate evolutionary branch, and was parallel to other related genera of Its phylogenetic distinctiveness and distinguishing phenotypic characteristics supported that strain T1lg56 represents a novel genus of the family , for which the name gen. nov., sp. nov. is proposed. The type strain is T1lg56 (=CCTCC AB 2016238=KCTC 52623).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): mangrove forest sediment , Mangrovicoccus ximenensis and Rhodobacteraceae
© 2018 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002796
2018-07-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/68/7/2172.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002796&mimeType=html&fmt=ahah

References

  1. Ghosh A, Dey N, Bera A, Tiwari A, Sathyaniranjan KB et al. Culture independent molecular analysis of bacterial communities in the mangrove sediment of Sundarban, India. Saline Systems 2010; 6:1 [View Article][PubMed]
     [Google Scholar]
  2. Garrity G, Brenner DJ, Kreig N, Staley JT. Bergey's Manual of Systematic Bacteriology, Vol. 2, Part C (The Alpha-, Beta-, Delta-, and Epsilonproteobacteria) New York: Springer; 2005 pp. 161–229
     [Google Scholar]
  3. Choi DH, Cho BC. Citreimonas salinaria gen. nov., sp. nov., a member of the Roseobacter clade isolated from a solar saltern. Int J Syst Evol Microbiol 2006; 56:2799–2803 [View Article][PubMed]
     [Google Scholar]
  4. Li S, Tang K, Liu K, Jiao N. Thiobacimonas profunda gen. nov., sp. nov., a member of the family Rhodobacteraceae isolated from deep-sea water. Int J Syst Evol Microbiol 2015; 65:359–364 [View Article][PubMed]
     [Google Scholar]
  5. Yang Y, Sun J, Tang K, Lin D, Li C et al. Ponticoccus lacteus sp. nov. of the family Rhodobacteraceae, isolated from surface seawater. Int J Syst Evol Microbiol 2015; 65:1247–1250 [View Article][PubMed]
     [Google Scholar]
  6. Dong XZ, Cai MY. Common Manual of Systematic Bacteriology Beijing: Science Press; 2001
     [Google Scholar]
  7. Lee MJ, Lee SS. Paracoccus limosus sp. nov., isolated from activated sludge in a sewage treatment plant. Int J Syst Evol Microbiol 2013; 63:1311–1316 [View Article][PubMed]
     [Google Scholar]
  8. Pujalte MJ, Lucena T, Ruvira MA, Arahal DR, Macián MC et al. The family Rhodobacteraceae. The Prokaryotes – Alphaproteobacteria and Betaproteobacteria Berlin: Springer Verlag; 2014 pp. 439–512
     [Google Scholar]
  9. Romanenko LA, Tanaka N, Svetashev VI, Kalinovskaya NI. Poseidonocella pacifica gen. nov., sp. nov. and Poseidonocella sedimentorum sp. nov., novel alphaproteobacteria from the shallow sandy sediments of the Sea of Japan. Arch Microbiol 2012; 194:113–121 [View Article][PubMed]
     [Google Scholar]
  10. Hameed A, Shahina M, Lin SY, Nakayan P, Liu YC et al. Youngimonas vesicularis gen. nov., sp. nov., of the family Rhodobacteraceae, isolated from surface seawater, reclassification of Donghicola xiamenensis Tan et al. 2009 as Pseudodonghicola xiamenensis gen. nov., comb. nov. and emended description of the genus Donghicola Yoon et al. 2007. Int J Syst Evol Microbiol 2014; 64:2729–2737 [View Article][PubMed]
     [Google Scholar]
  11. Wang B, Tan T, Shao Z. Roseovarius pacificus sp. nov., isolated from deep-sea sediment. Int J Syst Evol Microbiol 2009; 59:1116–1121 [View Article][PubMed]
     [Google Scholar]
  12. Yu Z, Wang J, Lin J, Zhao M, Qiu J. Exploring regulation genes involved in the expression of L-amino acid oxidase in Pseudoalteromonas sp. Rf-1. PLoS One 2015; 10:e0122741 [View Article][PubMed]
     [Google Scholar]
  13. Yu Z. Optimization of PCR amplification for sensitive capture of methanopyrus isoleucyl-tRNA synthetase gene in environmental samples. Ann Microbiol 2010; 60:757–762 [View Article]
     [Google Scholar]
  14. Yuan N, Zeng Y, Feng H, Yu Z, Huang Y. Altererythrobacter xixiisoli sp. nov., isolated from wetland soil. Int J Syst Evol Microbiol 2017; 67:3655–3659 [View Article][PubMed]
     [Google Scholar]
  15. Yu Z, Guo C, Qiu J. Precursor amino acids inhibit polymyxin E biosynthesis in Paenibacillus polymyxa, probably by affecting the expression of polymyxin E biosynthesis-associated genes. Biomed Res Int 2015; 2015:1–11 [View Article][PubMed]
     [Google Scholar]
  16. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol 1990; 215:403–410 [View Article][PubMed]
     [Google Scholar]
  17. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012; 62:716–721 [View Article][PubMed]
     [Google Scholar]
  18. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; 30:2725–2729 [View Article][PubMed]
     [Google Scholar]
  19. Yu Z, Ma Y, Zhong W, Qiu J, Li J. Comparative genomics of Methanopyrus sp. SNP6 and KOL6 revealing genomic regions of plasticity implicated in extremely thermophilic profiles. Front Microbiol 2017; 8:1278 [View Article][PubMed]
     [Google Scholar]
  20. Hu J, Zhang WY, Zhang XQ, Hong C, Zhu XF et al. Muriicola marianensis sp. nov., isolated from seawater. Int J Syst Evol Microbiol 2015; 65:407–411 [View Article][PubMed]
     [Google Scholar]
  21. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425 [View Article][PubMed]
     [Google Scholar]
  22. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article][PubMed]
     [Google Scholar]
  23. Kimura M, Kim OS, Cho YJ, Lee K, Yoon SH, Kim M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16:111–120[PubMed]
     [Google Scholar]
  24. Jeong SH, Park MS, Jin HM, Lee K, Park W et al. Aestuariibaculum suncheonense gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from a tidal flat and emended descriptions of the genera Gaetbulibacter and Tamlana. Int J Syst Evol Microbiol 2013; 63:332–338 [View Article][PubMed]
     [Google Scholar]
  25. Smibert RM, Krieg NR. Phenotypic characterization. Methods for General and Molecular Bacteriology Washington, DC: American Society of Microbiology; 1994 pp. 607–654
     [Google Scholar]
  26. Leifson E. Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 1963; 85:1183–1184[PubMed]
     [Google Scholar]
  27. Yurkov V, Stackebrandt E, Holmes A, Fuerst JA, Hugenholtz P et al. Phylogenetic positions of novel aerobic, bacteriochlorophyll a-containing bacteria and description of Roseococcus thiosulfatophilus gen. nov., sp. nov., Erythromicrobium ramosum gen. nov., sp. nov., and Erythrobacter litoralis sp. nov. Int J Syst Bacteriol 1994; 44:427–434 [View Article][PubMed]
     [Google Scholar]
  28. Folch J, Lees M, Sloane Stanley GH. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem 1957; 226:497–509[PubMed]
     [Google Scholar]
  29. Collins MD, Shah HN. Fatty acid, menaquinone and polar lipid composition of Rothia dentocariosa. Arch Microbiol 1984; 137:247–249 [View Article]
     [Google Scholar]
  30. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: Microbial ID Inc; 2001
     [Google Scholar]
  31. Komagata K, Susuki K. Lipid and cell-wall systematics in bacterial systematics. Method Microbiol 1987; 19:161–207
     [Google Scholar]
  32. Tindall BJ. Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 1990; 66:199–202 [View Article]
     [Google Scholar]
  33. Yoon JH, Kang SJ, Oh TK. Donghicola eburneus gen. nov., sp. nov., isolated from seawater of the East Sea in Korea. Int J Syst Evol Microbiol 2007; 57:73–76 [View Article][PubMed]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.002796
Loading
Mangrovicoccus ximenensis gen. nov., sp. nov., isolated from mangrove forest sediment
Int J Syst Evol Microbiol 68, 2172 (2018); https://doi.org/10.1099/ijsem.0.002796
/content/journal/ijsem/10.1099/ijsem.0.002796
/content/journal/ijsem/10.1099/ijsem.0.002796
Loading

Data & Media loading...

Supplements

Supplementary File 2

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error