1887

Abstract

An obligately anaerobic, Gram-positive, non-spore-forming, straight rod-shaped bacterium, designated strain 3BBH22, was isolated from a faecal sample of a healthy Japanese woman. The 16S rRNA gene sequence analysis showed that strain 3BBH22 formed a monophyletic cluster with species in the genera and within the family and had highest similarity to ATCC 29799 (96.7 % sequence similarity), followed by ATCC 29863 (96.4 %). Acetate and butyrate were produced by strain 3BBH22 as metabolic end-products. The major cellular fatty acids were C, C, Cω9, C dimethyl acetal, C and Cω6,9. No respiratory quinones were detected. In contrast to JCM 32125, strain 3BBH22 did not degrade quercetin, one of the flavonoids. JCM 32126 also did not. Strain 3BBH22 was differentiated from JCM 32126 by its inability to hydrolyse aesculin. The G+C content of the genomic DNA was 61.2±1.0 mol%. On the basis of these data and the phylogenetic tree based on 89 proteins, strain 3BBH22 represents a novel species in a novel genus of the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is 3BBH22 (=JCM 32166=DSM 106493).

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2018-06-01
2024-03-28
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