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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 68, Issue 7

gen. nov., sp. nov., an -alkane-degrading bacterium isolated from seawater Free

Abstract

Strain GI5 was isolated from a surface seawater sample collected from Garorim Bay (West Sea, Republic of Korea). The isolated strain was aerobic, Gram-stain-negative, rod-shaped, motile by means of a polar flagellum, negative for catalase and weakly positive for oxidase. The optimum growth pH, salinity and temperature were determined to be pH 7.5–8.0, 3 % NaCl (w/v) and 25 °C, respectively; the growth ranges were pH 6.0–9.0, 1–7 % NaCl (w/v) and 18–40 °C. The results of phylogenetic analysis of 16S rRNA gene sequences indicated that GI5 clustered within the family and most closely with B-5 and R8-12 (91.9 % and 91.6 % similarity, respectively). The major cellular fatty acids in GI5 were Cω7/Cω6 (44.45 %), Cω6/Cω7 (14.17 %) and C (10.19 %); this profile was distinct from those of the closely related species. The major respiratory quinone of GI5 was Q-8. The main polar lipids were phosphatidylethanolamine and phosphatidylglycerol. Two putative alkane hydroxylase () genes were identified in GI5. The G+C content of the genomic DNA of GI5 was determined to be 51.2 mol%. On the basis of the results of phenotypic, chemotaxonomic and phylogenetic studies, strain GI5 represents a novel species of a novel genus of the family for which we propose the name gen. nov., sp. nov.; the type strain is GI5 (=KCTC 52659=JCM 31835).

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Keyword(s): Alcanivoracaceae , Ketobacter alkanivorans and n-alkane degradation
© 2018 IUMS
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2018-07-01
2024-04-25
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Ketobacter alkanivorans gen. nov., sp. nov., an n-alkane-degrading bacterium isolated from seawater
Int J Syst Evol Microbiol 68, 2258 (2018); https://doi.org/10.1099/ijsem.0.002823
/content/journal/ijsem/10.1099/ijsem.0.002823
/content/journal/ijsem/10.1099/ijsem.0.002823
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