1887

Abstract

Two strains, which formed pink colonies and produced ballistoconidia and represented a novel anamorphic yeast species, were isolated from peat (DMKU-SPS1-2) and fern leaf (ST-145) collected in Thailand. Analysis of the sequences of the D1/D2 domains of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) regions showed that the two strains were identical to the sequences of the D1/D2 domains of the LSU rRNA gene and differed by two nucleotide substitutions in the ITS regions. Phylogenetic analysis based on the combined sequences of the ITS and the D1/D2 regions confirmed that the two strains represented a single species in the genus Cryptotrichosporon that was distinct from the other known species of the genus. Cryptotrichosporon argae (CBS 14376) was the most closely related species, but with 2.2 % nucleotide substitutions in the D1/D2 domains of the LSU rRNA gene, and 6.8–8.0 % nucleotide substitutions in the ITS regions. Therefore, the two strains were assigned as a novel species, for which we propose the name Cryptotrichosporon siamense sp. nov. The type is DMKU-SPS1-2. The MycoBank number of the novel species is MB82336.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002858
2018-06-19
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/68/8/2473.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002858&mimeType=html&fmt=ahah

References

  1. Okoli I, Oyeka CA, Kwon-Chung KJ, Theelen B, Robert V et al. Cryptotrichosporon anacardii gen. nov., sp. nov., a new trichosporonoid capsulate basidiomycetous yeast from Nigeria that is able to form melanin on niger seed agar. FEMS Yeast Res 2007; 7:339–350 [View Article][PubMed]
    [Google Scholar]
  2. Wang L, Wang QM. Molecular phylogenetic analysis of ballistoconidium-forming yeasts in Trichosporonales (Tremellomycetes): a proposal for Takashimella gen. nov. and Cryptotrichosporon tibetense sp. nov. PLoS One 2015; 10:e0132653 [View Article][PubMed]
    [Google Scholar]
  3. Pontes A, Röhl O, Maldonado C, Yurkov AM, Sampaio JP. Cryptotrichosporon argae sp. nov., Cryptotrichosporon brontae sp. nov. and Cryptotrichosporon steropae sp. nov., isolated from forest soils. Int J Syst Evol Microbiol 2017; 67:3610–3614 [View Article][PubMed]
    [Google Scholar]
  4. Liu XZ, Wang QM, Göker M, Groenewald M, Kachalkin AV et al. Towards an integrated phylogenetic classification of the Tremellomycetes. Stud Mycol 2015; 81:85–147 [View Article][PubMed]
    [Google Scholar]
  5. Liu XZ, Wang QM, Theelen B, Groenewald M, Bai FY et al. Phylogeny of tremellomycetous yeasts and related dimorphic and filamentous basidiomycetes reconstructed from multiple gene sequence analyses. Stud Mycol 2015; 81:1–26 [View Article][PubMed]
    [Google Scholar]
  6. Posa MRC, Wijedasa LS, Corlett RT. Biodiversity and conservation of tropical peat swamp forests. BioScience 2011; 61:49–57 [View Article]
    [Google Scholar]
  7. Thormann MN, Rice AV, Beilman DW. Yeasts in peatlands: a review of richness and roles in peat decomposition. Wetlands 2007; 27:761–773 [View Article]
    [Google Scholar]
  8. Kachalkin AV, Glushakova AM, Iurkov AM, Chernov N. Characterization of yeast groupings in the phyllosphere of Sphagnum mosses. Mikrobiologiia 2008; 77:474–481 [View Article][PubMed]
    [Google Scholar]
  9. Kachalkin AV, Yurkov AM. Yeast communities in Sphagnum phyllosphere along the temperature-moisture ecocline in the boreal forest-swamp ecosystem and description of Candida sphagnicola sp. nov. Antonie van Leeuwenhoek 2012; 102:29–43 [View Article][PubMed]
    [Google Scholar]
  10. Grum-Grzhimaylo OA, Debets AJ, Bilanenko EN. The diversity of microfungi in peatlands originated from the White Sea. Mycologia 2016; 108:233–254 [View Article][PubMed]
    [Google Scholar]
  11. Jaiboon K, Lertwattanasakul N, Limtong P, Limtong S. Yeasts from peat in a tropical peat swamp forest in Thailand and their ability to produce ethanol, indole-3-acetic acid and extracellular enzymes. Mycol Prog 2016; 15:755–770 [View Article]
    [Google Scholar]
  12. Polburee P, Lertwattanasakul N, Limtong P, Groenewald M, Limtong S. Nakazawaea todaengensis f.a., sp. nov., a yeast isolated from a peat swamp forest in Thailand. Int J Syst Evol Microbiol 2017; 67:2377–2382 [View Article][PubMed]
    [Google Scholar]
  13. Nakase T, Tsuzuki S, Lee FL, Jindamorakot S, Jan-Ngam H et al. Sporobolomyces diospyroris sp. nov., Sporobolomyces lophatheri sp. nov. and Sporobolomyces pyrrosiae sp. nov., three new species of ballistoconidium-forming yeasts in the Agaricostilbum lineage isolated from plants in Taiwan. J Gen Appl Microbiol 2005; 51:277–286 [View Article][PubMed]
    [Google Scholar]
  14. Kurtzman CP, Robnett CJ. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 1998; 73:331–371 [View Article][PubMed]
    [Google Scholar]
  15. White TJ, Bruns T, Lee S, Taylor JW. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In Innis MA, Gelfand DH, Sninsky JJ, White TJ. (editors) PCR Protocols: A Guide to Methods and Applications New York: Academic Press; 1990 pp. 315–322
    [Google Scholar]
  16. Limtong S, Yongmanitchai W, Tun MM, Kawasaki H, Seki T. Kazachstania siamensis sp. nov., an ascomycetous yeast species from forest soil in Thailand. Int J Syst Evol Microbiol 2007; 57:419–422 [View Article][PubMed]
    [Google Scholar]
  17. Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z et al. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 1997; 25:3389–3402 [View Article][PubMed]
    [Google Scholar]
  18. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article][PubMed]
    [Google Scholar]
  19. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article][PubMed]
    [Google Scholar]
  20. Kurtzman CP, Fell JW, Boekhout T, Robert V. Methods for isolation, phenotypic characterization and maintenance of yeasts. In Kurtzman CP, Fell JW, Boekhout T. (editors) The Yeasts, A Taxonomic Study, 5th ed. Amsterdam: Elsevier; 2011 pp. 87–110
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.002858
Loading
/content/journal/ijsem/10.1099/ijsem.0.002858
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error