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Volume 68, Issue 8

Sphingomonas tabacisoli sp. nov., a member of the genus Sphingomonas, isolated from rhizosphere soil of Nicotiana tabacum L. Free

Abstract

A Gram-staining-negative, aerobic, motile and rod-shaped bacterium, designated strain X1-8, was isolated from rhizosphere soil of Nicotiana tabacum L. collected from the tobacco produce base located in Kunming, south-west PR China. Cells showed oxidase-negative and catalase-positive reactions and were motile by means of peritrichous flagella. Growth occurred at 25–40 °C and pH 6.0–8.0 with optimal growth at 30–35 °C, pH 7.0. The major respiratory lipoquinone was Q-10. C16 : 0 and summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) were identified as major cellular fatty acids. The profile of polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, sphingoglycolipid, phosphatidylcholine and one unidentified glycolipid. The major polyamine was sym-homospermidine. The genomic DNA G+C content was 66.5 mol%. The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that X1-8 should be affiliated to the genus Sphingomonas and formed a clade with most closely related species Sphingomonas changbaiensis NBRC 104936. The results of 16S rRNA gene sequences similarity analysis indicated that X1-8 had the highest similarity with S. changbaiensis NBRC 104936 (98.4 %) and lower than 96.0 % with other species of the genus Sphingomonas . DNA–DNA hybridization data indicated that X1-8 represented a novel genomic species of the genus Sphingomonas . The characteristics determined in the polyphasic taxonomic study indicated that X1-8 represents a novel species of the genus Sphingomonas , for which the name Sphingomonas tabacisoli sp. nov. (type strain X1-8=KCTC 62032=CGMCC 1.16275) is proposed.

  • Received:
  • Accepted:
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Keyword(s): Nicotiana tabacum L , polyphasic taxonomy , rhizosphere soil and Sphingomonas tabacisoli sp. nov.
© 2018 IUMS
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2018-06-26
2024-04-18
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Sphingomonas tabacisoli sp. nov., a member of the genus Sphingomonas, isolated from rhizosphere soil of Nicotiana tabacum L.
Int J Syst Evol Microbiol 68, 2574 (2018); https://doi.org/10.1099/ijsem.0.002879
/content/journal/ijsem/10.1099/ijsem.0.002879
/content/journal/ijsem/10.1099/ijsem.0.002879
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